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In vitro photodynamic inactivation effects of hypocrellin B on azole-sensitive and resistant Candida albicans
Jan, Asif; Liu, Chengcheng; Deng, Hong; Li, Jiao; Ma, Wenpeng; Zeng, Xiaoyan; Ji, Yanhong
2019-09-01
Source PublicationPHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
ISSN1572-1000
Volume27Pages:419-427
AbstractBackground and aim: The extensive use of antifungal drugs has led to resistance from Candida albicans. The search for alternative treatment against drug-resistant C. albicans is highly desirable. Antimicrobial photodynamic therapy (aPDT) is an emerging and promising approach for treating localized and superficial C. albicans infections. The aim of this study was to investigate the photodynamic inactivation (PDI) effects of hypocrellin B (HB) on azole-sensitive and resistant C. albicans in vitro.Methods: The PDI efficacies of HB on standard C. albicans strain (ATCC 10231), azole-sensitive clinical isolate of C. albicans, and azole-resistant clinical isolate of C. albicans were assessed. The uptake of HB in C. albicans cells was investigated by confocal laser scanning microscopy (CLSM). The PDI effects on cellular structure and surface characteristics were investigated by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).Results: HB exhibited no significant dark toxicity, but inactivated the azole-sensitive and resistant C. albicans in a light-dose and PS concentration-dependent manner. CLSM images indicated that PDI treated C. albicans cells showed stronger fluorescence compared to untreated cells. TEM images suggested that significant damage to the cell wall, membrane, and cytoplasm were induced by HB-mediated PDI. SEM analysis revealed that the surface of C. albicans cells became twisted and ruptured after PDI treatment.Conclusions: Azole-sensitive and resistant C. albicans could be effectively inactivated by HB in the presence of light, and HB-mediated aPDT shows promise as an antifungal treatment for C. albicans.
DOI10.1016/j.pdpdt.2019.07.014
Indexed BySCI
Language英语
WOS IDWOS:000484870600069
EI Accession Number1873-1597
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/82613
Collection中国科学院化学研究所
AffiliationChinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Jan, Asif,Liu, Chengcheng,Deng, Hong,et al. In vitro photodynamic inactivation effects of hypocrellin B on azole-sensitive and resistant Candida albicans[J]. PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY,2019,27:419-427.
APA Jan, Asif.,Liu, Chengcheng.,Deng, Hong.,Li, Jiao.,Ma, Wenpeng.,...&Ji, Yanhong.(2019).In vitro photodynamic inactivation effects of hypocrellin B on azole-sensitive and resistant Candida albicans.PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY,27,419-427.
MLA Jan, Asif,et al."In vitro photodynamic inactivation effects of hypocrellin B on azole-sensitive and resistant Candida albicans".PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY 27(2019):419-427.
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