ICCAS OpenIR
A label-free colorimetric detection of microRNA via hG-quadruplex-based signal quenching strategy
Lan, Ling; Wang, Rui-Li; Liu, Li; Cheng, Liang
2019-11-04
Source PublicationANALYTICA CHIMICA ACTA
ISSN0003-2670
Volume1079Pages:207-211
AbstractSignal amplification strategy have been the intensive direction for highly-sensitive microRNA (miRNA) detection, however, it is worth noting that signal quenching approach is rarely realized. Inspired by the unwinding of G-quadruplex with Klenow Fragment polymerase (KF), a simple and label-free signal quenching system for detection of miRNAs was developed. In the presence of target miRNA, miRNA was used as a primer to promote KF activity resulting in the disruption of G-quadruplex structure of probe and the biological catalysis inactivation of DNAzyme formed by G-quadruplex structure and hemin. As a result, the probe would be transformed from G-quadruplex to duplex and the obvious differential signal could be observed in most cases even with naked eyes. With the significant signal decreasing, the target miRNA can be rapidly analyzed by UVevis spectra with a considerably low detection limit (4.5 nM). This method exhibits excellent selectivity and anti-interference ability by discriminating base-mismatched and other miRNA families from target miRNA. Meanwhile, a good recovery (90.7%similar to 102.4%) in 5% human serum was obtained and this strategy verified a high expression level of miR21 in total RNA of MCF-7 compared with 293T and HeLa. It implies that this assay is of great potential to be applied in biochemical research and clinical diagnosis. (C) 2019 Elsevier B.V. All rights reserved.
DOI10.1016/j.aca.2019.06.063
Indexed BySCI
Language英语
WOS IDWOS:000478681100021
EI Accession Number1873-4324
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/82574
Collection中国科学院化学研究所
AffiliationChinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Lan, Ling,Wang, Rui-Li,Liu, Li,et al. A label-free colorimetric detection of microRNA via hG-quadruplex-based signal quenching strategy[J]. ANALYTICA CHIMICA ACTA,2019,1079:207-211.
APA Lan, Ling,Wang, Rui-Li,Liu, Li,&Cheng, Liang.(2019).A label-free colorimetric detection of microRNA via hG-quadruplex-based signal quenching strategy.ANALYTICA CHIMICA ACTA,1079,207-211.
MLA Lan, Ling,et al."A label-free colorimetric detection of microRNA via hG-quadruplex-based signal quenching strategy".ANALYTICA CHIMICA ACTA 1079(2019):207-211.
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