ICCAS OpenIR
Chiral separation and enantiomeric purity determination of pazufloxacin mesilate by HPLC using chiral mobile phase additives
Zhang, ZF; Yang, GL; Wang, DX; Liang, GJ; Chen, Y
2004
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
Volume27Issue:5Pages:813-827
AbstractIn this work, a chiral separation method based on an ODS column and chiral mobile phase additives (CMPA) for a new fluoroquinolones drug pazufloxacin mesilate [(-)-(S)-10-(1-amino-cyclopropyl)-9-fluoro-3-methyl-7-oxo-2,3-dihydro-7H-pyrido(1,2,3-de)-1,4-benzoxazine-6-carboxylic acid mesilate] and its R-(+)-enantiomer was developed. The chiral separation was performed on a Shimadzu CLC-ODS column (150 x 6.3 mm, 10 mum), methanol-phenylalanine solution (containing 8 mmol L-1 L-phenylalanine and 6.3 mmol L-1 copper sulfate, adjusted to pH = 3.5 with sodium hydroxide solution) (30: 70) was used as the chiral mobile phase and detected at 332 nm. Under the above conditions, the two enantiomers could be baseline separated with a resolution 1.9 and the chiral purity was quantitatively determined. Calibration graphs were constructed in the range of 2.48-248 mug mL(-1) for S-(-)- and 2.52-252 mug mL(-1) for R-(+)-enantiomer, respectively. The linear correlation equations were: Y = 1.38 x 10(5)X - 130.9 (r = 0.9998) for S-(-)- and Y = 1.26 x 10(5)X - 60.6 (r = 0.9998) for R-(+) enantiomer, respectively. The detection limits were 1.2 ng for S-(-)- and 1.5 ng for R-(+)- enantiomer(obtained by SIN = 3), respectively. The RSD of the method was below 1.5% (n = 5).
KeywordChiral Mobile Phase Additives Chiral Separation Phase L-phenylalanine R-and S-enantiomers
DOI10.1081/JLC-120029700
Indexed BySCI
Language英语
WOS IDWOS:000220591700004
PublisherMARCEL DEKKER INC
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/82568
Collection中国科学院化学研究所
Corresponding AuthorYang, GL
Affiliation1.Hebei Univ, Coll Chem & Environm Sci, Baoding 071002, Peoples R China
2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
3.Gen Hosp AF PLA, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhang, ZF,Yang, GL,Wang, DX,et al. Chiral separation and enantiomeric purity determination of pazufloxacin mesilate by HPLC using chiral mobile phase additives[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2004,27(5):813-827.
APA Zhang, ZF,Yang, GL,Wang, DX,Liang, GJ,&Chen, Y.(2004).Chiral separation and enantiomeric purity determination of pazufloxacin mesilate by HPLC using chiral mobile phase additives.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,27(5),813-827.
MLA Zhang, ZF,et al."Chiral separation and enantiomeric purity determination of pazufloxacin mesilate by HPLC using chiral mobile phase additives".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 27.5(2004):813-827.
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