ICCAS OpenIR
Chiral separation of nateglinide and its (L) enantiomer on a molecularly imprinted polymer-based stationary phase
Yang, GL; Yin, JF; Li, ZW; Liu, HY; Cai, LP; Wang, DX; Chen, Y
2004-06-01
Source PublicationCHROMATOGRAPHIA
ISSN0009-5893
Volume59Issue:11-12Pages:705-708
AbstractA new chiral stationary phase for nateglinide (N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine) based on a molecularly imprinted polymer has been prepared by non-covalent imprinting. For chromatographic analysis the effects on the separation of mobile phase composition, flow rate, and temperature were investigated, and the optimum conditions for HPLC were shown to be: mobile phase, acetonitrile; flow rate, 0.5 mL min(-1); temperature, 25 degreesC. It was shown that the noteglinide-imprinted polymer was capable of recognizing the enantiomeric difference between nateglinide and its L enantiomer, whereas the non-imprinted polymer had no such ability. Scatchard analysis was used to investigate the binding characteristics of the noteglinide-imprinted stationary phase; this indicated that two classes of binding site were present in the imprinted polymer. The equilibrium dissociation constant (K-D) and the apparent maximum number (Q(max)) of high- and low-affinity binding sites were 3.7 x 10(-4) mol L-1 and 11.38 mumol g(-1), and 1.81 x 10(-3) mol L-1 and 27.73 mumol g(-1), respectively.
KeywordColumn Liquid Chromatography Molecularly Imprinted Stationary Phase Chiral Separation Nateglinide
DOI10.1365/s10337-004-0276-y
Indexed BySCI
Language英语
WOS IDWOS:000224100300008
PublisherVIEWEG
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/82412
Collection中国科学院化学研究所
Corresponding AuthorYang, GL
Affiliation1.Hebei Univ Baoding, Coll Pharm, Baoding 071002, Peoples R China
2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yang, GL,Yin, JF,Li, ZW,et al. Chiral separation of nateglinide and its (L) enantiomer on a molecularly imprinted polymer-based stationary phase[J]. CHROMATOGRAPHIA,2004,59(11-12):705-708.
APA Yang, GL.,Yin, JF.,Li, ZW.,Liu, HY.,Cai, LP.,...&Chen, Y.(2004).Chiral separation of nateglinide and its (L) enantiomer on a molecularly imprinted polymer-based stationary phase.CHROMATOGRAPHIA,59(11-12),705-708.
MLA Yang, GL,et al."Chiral separation of nateglinide and its (L) enantiomer on a molecularly imprinted polymer-based stationary phase".CHROMATOGRAPHIA 59.11-12(2004):705-708.
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