Selective labeling of histidine by a designed fluorescein-based probe
Li, XH; Ma, HM; Dong, SY; Duan, XJ; Liang, SC
Source PublicationTALANTA
AbstractThe synthesis of a novel fluorescent probe, 3-epoxypropoxy fluorescein (EPF), and its properties for labeling of histidine are described. The probe contained a fluorescein fluorophore with long-wavelength response and an active epoxy labeling group. In alkaline media EPF reacted selectively with histidine, rather than with other amino acids, causing a large increase in fluorescence intensity and thereby allowing a selective detection of histidine. This fluorescence increase resembled that of the fluorescein diaion with the increase of the media basicity, suggesting that the addition reaction of histidine with the epoxy group provides the fluorophore moiety with a basic molecular environment. As an application of this probe, fluorescent labeling of histidine in human serum was attempted and the obtained results were in agreement. with those given by using histidine-nickel complex adsorptive voltammetry. Further, the relative S.D. of the method was 1.8% for 10 replicate determinations of 0.55 muM histidine. When 10 muM of EPF was used, the linear range for histidine was 0.007-10 muM with a detection limit (S/N = 3) of 0.001 muM. (C) 2003 Elsevier B.V. All rights reserved.
KeywordFluorescent Probe 3-epoxypropoxy Fluorescein Histidine Labeling
Indexed BySCI
WOS IDWOS:000187955900020
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Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorMa, HM
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Biol Chem Lab, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Li, XH,Ma, HM,Dong, SY,et al. Selective labeling of histidine by a designed fluorescein-based probe[J]. TALANTA,2004,62(2):367-371.
APA Li, XH,Ma, HM,Dong, SY,Duan, XJ,&Liang, SC.(2004).Selective labeling of histidine by a designed fluorescein-based probe.TALANTA,62(2),367-371.
MLA Li, XH,et al."Selective labeling of histidine by a designed fluorescein-based probe".TALANTA 62.2(2004):367-371.
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