ICCAS OpenIR
Direct visualization of the dynamic hydrolysis process of an L-DPPC monolayer catalyzed by phospholipase D at the air/water interface
He, Q; Zhai, XH; Li, JB
2004-01-08
Source PublicationJOURNAL OF PHYSICAL CHEMISTRY B
ISSN1520-6106
Volume108Issue:1Pages:473-476
AbstractThe hydrolysis reaction of L-alpha- dipalmitoylphosphatidylcholine (L-DPPC) catalyzed by cabbage phospholipase D (PLD) at the air/water interface has been studied by Brewster angle microscopy (BAM) and film balance technique. The curves of surface pressure versus time show that the hydrolysis reaction depends on the initial state of the lipid monolayer. The BAM images display that the hydrolysis reaction preferentially occurs in the liquid-expanded phase where PLD has a maximum activity. In the coexistence region of liquid-expanded and liquid-condensed phases, a "lotus-like" domain of the monolayer was observed, indicating that the hydrolysis product 1, 2-dipalmitoylphosphatidic acid (DPPA) created a new phase, which inhibited the reaction rate of hydrolysis.
DOI10.1021/jp036071c
Indexed BySCI
Language英语
WOS IDWOS:000187838800063
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81866
Collection中国科学院化学研究所
Corresponding AuthorLi, JB
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Key Lab Colloid & Interface Sci,Int Joint Lab, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
He, Q,Zhai, XH,Li, JB. Direct visualization of the dynamic hydrolysis process of an L-DPPC monolayer catalyzed by phospholipase D at the air/water interface[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2004,108(1):473-476.
APA He, Q,Zhai, XH,&Li, JB.(2004).Direct visualization of the dynamic hydrolysis process of an L-DPPC monolayer catalyzed by phospholipase D at the air/water interface.JOURNAL OF PHYSICAL CHEMISTRY B,108(1),473-476.
MLA He, Q,et al."Direct visualization of the dynamic hydrolysis process of an L-DPPC monolayer catalyzed by phospholipase D at the air/water interface".JOURNAL OF PHYSICAL CHEMISTRY B 108.1(2004):473-476.
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