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Structural changes of phospholipid monolayers caused by coupling of human serum albumin: A GIXD study at the air/water interface
Wang, XL; He, Q; Zheng, SP; Brezesinski, G; Mohwald, H; Li, JB
2004-09-16
Source PublicationJOURNAL OF PHYSICAL CHEMISTRY B
ISSN1520-6106
Volume108Issue:37Pages:14171-14177
AbstractPhase behavior and structural changes were studied for phospholipid monolayers coupled with human serum albumin (HSA) at the air/buffer (pH 3.8) interface by film balance and grazing incidence X-ray diffraction (GIXD) measurements. For comparison, the lipid headgroup was varied from small ionic L-alpha-dipalmitoylphosphatidic acid (DPPA) to large ionic L-alpha-dipalmitoyl-phosphatidyl-L-serine (DPPS) and zwitterionic L-alpha-distearoyl-phosphatidylcholine (DSPC). The presence of HSA leads to a more or less pronounced plateau in the pressure/area isotherms of all phospholipids. GIXD diffraction patterns illustrate that the phase sequences of DPPS and DPPA on buffer (oblique-rectangular-hexagonal) are changed because of the binding of HSA. The oblique-rectangular phase transition disappears and the rectangular-hexagonal phase transition shifts to a lower surface pressure. However, there is no effect of HSA on the structure of the DSPC monolayer (NN tilted rectangular packing). Phase diagrams of DPPA/HSA, DPPS/HSA, and DSPC/HSA have been established. A tilt angle decrease in both DPPA/HSA and DPPS/HSA monolayers is observed, but no change of the tilt angle for DSPC molecules in the mixed DSPC/HSA monolayer is found. Thus, whereas HSA binding stabilizes the liquid-expanded phase at low pressures most probably via penetration, it condenses the ordered phases of anionic monolayers via reducing the headgroup repulsion. The positional correlation lengths xi(i) parallel and perpendicular to the tilt direction change because of the binding of HSA.
DOI10.1021/jp0470243
Indexed BySCI
Language英语
WOS IDWOS:000224164000040
PublisherAMER CHEMICAL SOC
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81836
Collection中国科学院化学研究所
Corresponding AuthorLi, JB
Affiliation1.Chinese Acad Sci, Ctr Mol Sci, Inst Chem, Int Joint Lab,Key Lab Colloid & Interface Sci, Beijing 100080, Peoples R China
2.Max Planck Inst Colloids & Interfaces, D-14476 Potsdam, Germany
Recommended Citation
GB/T 7714
Wang, XL,He, Q,Zheng, SP,et al. Structural changes of phospholipid monolayers caused by coupling of human serum albumin: A GIXD study at the air/water interface[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2004,108(37):14171-14177.
APA Wang, XL,He, Q,Zheng, SP,Brezesinski, G,Mohwald, H,&Li, JB.(2004).Structural changes of phospholipid monolayers caused by coupling of human serum albumin: A GIXD study at the air/water interface.JOURNAL OF PHYSICAL CHEMISTRY B,108(37),14171-14177.
MLA Wang, XL,et al."Structural changes of phospholipid monolayers caused by coupling of human serum albumin: A GIXD study at the air/water interface".JOURNAL OF PHYSICAL CHEMISTRY B 108.37(2004):14171-14177.
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