ICCAS OpenIR
A novel fluorescent probe for selective labeling of histidine
Li, XH; Ma, HM; Nie, LH; Sun, M; Xiong, SX
2004-07-12
Source PublicationANALYTICA CHIMICA ACTA
ISSN0003-2670
Volume515Issue:2Pages:255-260
AbstractA novel water-soluble fluorescent probe, 1-(2,3-epoxypropoxy)-2-phenylazo-8-acetylamino-naphthalene-3,6-disulfonic acid sodium salt (EPANS), was designed and synthesized by reacting epichlorohydrin with azophloxine, and its properties for labeling of histidine were studied. In alkaline media, EPANS reacted selectively with histidine rather than with other amino acids, causing a large decrease in fluorescence intensity and thereby allowing a selective detection of histidine without a tedious separation. The linear range was 4-500 muM with a detection limit of 1 muM (S/N = 3). The proposed method has been successfully used to determine histidine in human serum. The obtained results were in good agreement with those given by using histidine-nickel complex adsorptive voltammetry. (C) 2004 Elsevier B.V. All rights reserved.
KeywordFluorescent Probe Histidine Labeling 1-(2 3-epoxypropoxy)-2-phenylazo-8-acetylaminonaphthalene-3 6-disulfonic Acid Sodium Salt
DOI10.1016/j.aca.2004.03.008
Indexed BySCI
Language英语
WOS IDWOS:000222370100002
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81696
Collection中国科学院化学研究所
Corresponding AuthorMa, HM
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Biol Chem Lab, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Li, XH,Ma, HM,Nie, LH,et al. A novel fluorescent probe for selective labeling of histidine[J]. ANALYTICA CHIMICA ACTA,2004,515(2):255-260.
APA Li, XH,Ma, HM,Nie, LH,Sun, M,&Xiong, SX.(2004).A novel fluorescent probe for selective labeling of histidine.ANALYTICA CHIMICA ACTA,515(2),255-260.
MLA Li, XH,et al."A novel fluorescent probe for selective labeling of histidine".ANALYTICA CHIMICA ACTA 515.2(2004):255-260.
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