ICCAS OpenIR
Measuring specific interaction of transcription factor ZmDREB1A with its DNA responsive element at the molecular level
Jiang, YX; Qin, F; Li, YQ; Fang, XH; Bai, CL
2004-07-01
Source PublicationNUCLEIC ACIDS RESEARCH
ISSN0305-1048
Volume32Issue:12
AbstractSpecific Interactions between transcription factors and DNA responsive elements are of fundamental Importance in understanding how genetic regulatory proteins control gene transcription. Here we have developed a new method of using atomic force microscopy (AFM) to quantitatively study the single molecular specific Interaction between ZmDREB1A, a transcription factor from maize, and its DNA responsive element, dehydration-responsive element (DRE) with core sequence A/GCCGAC. It was found that ZmDREB1A bound to both DRE ACCGAC and GCCGAC efficiently. The single molecular Interaction forces of ZmDREB1A with DRE A/GCCGAC were determined to be 101 +/- 5 and 108 +/- 3 pN, respectively. The point mutation of ZmDREB1A in its DNA-binding domain or single base substitution of the DRE core sequence greatly reduced the binding affinity, demonstrating the high sensitivity of the AFM measurements. AFM is expected to be a simple, quick, sensitive and reliable method that offers valuable information for the characterization of transcription factors and the identification of their potential DNA responsive elements in functional genomics research.
DOI10.1093/nar/gnh100
Indexed BySCI
Language英语
WOS IDWOS:000223341900035
PublisherOXFORD UNIV PRESS
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81688
Collection中国科学院化学研究所
Corresponding AuthorFang, XH
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
2.Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
Jiang, YX,Qin, F,Li, YQ,et al. Measuring specific interaction of transcription factor ZmDREB1A with its DNA responsive element at the molecular level[J]. NUCLEIC ACIDS RESEARCH,2004,32(12).
APA Jiang, YX,Qin, F,Li, YQ,Fang, XH,&Bai, CL.(2004).Measuring specific interaction of transcription factor ZmDREB1A with its DNA responsive element at the molecular level.NUCLEIC ACIDS RESEARCH,32(12).
MLA Jiang, YX,et al."Measuring specific interaction of transcription factor ZmDREB1A with its DNA responsive element at the molecular level".NUCLEIC ACIDS RESEARCH 32.12(2004).
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