ICCAS OpenIR
Chromatic immunoassay based on polydiacetylene vesicles
Su, YL; Li, JR; Jiang, L
2004-10-10
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume38Issue:1-2Pages:29-33
AbstractA new approach of chromatic immunoassay based on polydiacetylene vesicles is described. Antibodies were covalently coupled with mixed vesicles of 40,12-tricosadiynoic acid (TCDA) and dimyristoylphosphaticlycholine (DMPC). The vesicle-antibody conjugates were irradiated with UV light to yield a blue-colored polydiacetylene. After antigen injection, specific immunoreactions took place at the vesicle surface alter polydiacetylene conformation and lead to a color change from blue to red. The chromatic immunoassay described here is simple, rapid, sensitive; the color change was readily discernible by naked eye when the concentration of antigen is 1 ng/mL. Incorporation of DMPC in the mixed vesicles increases the sensitivity of the chromatic immunoassay. (C) 2004 Elsevier B.V. All rights reserved.
KeywordChromatic Immunoassay Polydiacetylene Vesicles
DOI10.1016/j.colsurfb.2004.08.010
Indexed BySCI
Language英语
WOS IDWOS:000224672800005
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81630
Collection中国科学院化学研究所
Corresponding AuthorJiang, L
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Key Lab Colloid & Interface Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Su, YL,Li, JR,Jiang, L. Chromatic immunoassay based on polydiacetylene vesicles[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2004,38(1-2):29-33.
APA Su, YL,Li, JR,&Jiang, L.(2004).Chromatic immunoassay based on polydiacetylene vesicles.COLLOIDS AND SURFACES B-BIOINTERFACES,38(1-2),29-33.
MLA Su, YL,et al."Chromatic immunoassay based on polydiacetylene vesicles".COLLOIDS AND SURFACES B-BIOINTERFACES 38.1-2(2004):29-33.
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