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Novel biodegradable aliphatic poly(butylene succinate-co-cyclic carbonate)s bearing functionalizable carbonate building blocks: II. Enzymatic biodegradation and in vitro biocompatibility assay
Jing, Y; Tian, WS; Li, QB; Yang, L; Cao, AM
2004-11-01
Source PublicationBIOMACROMOLECULES
ISSN1525-7797
Volume5Issue:6Pages:2258-2268
AbstractIn a previous study, we have reported chemical synthesis of novel aliphatic poly(butylene succinate-co-cyclic carbonate) P(BS-co-CC)s bearing various functionalizable carbonate building blocks, and this work will continue to present our new studies on their enzymatic degradation and in vitro cell biocompatibility assay. First, enzymatic degradation of the novel P(BS-co-CC) film samples was investigated with two enzymes of lipase B Candida Antartic (Novozyme 435) and lipase Porcine Pancreas PPL, and it was revealed that copolymerizing linear poly(butylene succinate) PBS with a functionalizable carbonate building block could remarkably accelerate the enzymatic degradation of a synthesized product P(BS-co-CC), and its biodegradation behavior was found to strongly depend on the overall impacts of several important factors as the cyclic carbonate (CC) comonomer structure and molar content, molar mass, thermal characteristics, morphology, the enzyme-substrate specificity, and so forth. Further, the biodegraded residual film samples and watersoluble enzymatic degradation products were allowed to be analyzed by means of proton nuclear magnetic resonance (H-1 NMR), gel permeation chromatograph (GPC), differential scanning calorimeter (DSC), attenuated total reflection FTIR (ATR-FTIR), scanning electron microscope (SEM), and liquid chromatograph-mass spectrometry (LC-MS). On the experimental evidences, an exo-type mechanism of enzymatic chain hydrolysis preferentially occurring in the noncrystalline domains was suggested for the synthesized new P(BS-co-CC) film samples. With regard to their cell biocompatibilities, an assay with NIH 3T3 mouse fibroblast cell was conducted using the novel synthesized P(BS-co-CC) films as substrates with respect to the cell adhesion and proliferation, and these new biodegradable P(BS-co-CC) samples were found to exhibit as low cell toxicity as the PLLA control, particularly the two samples of poly(butylene succinate-co-18.7 mol % dimethyl trimethylene carbonate) P(BS-co-18.7 mol % DMTMC) and poly(butylene succinate-co-21.9 mol % 5-benzyloxy trimethylene carbonate) P(BS-co-21.9 mol % BTMC) were interestingly found to show much better cell biocompatibilities than the PLLA reference.
DOI10.1021/bm049705+
Indexed BySCI
Language英语
WOS IDWOS:000225091700022
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81572
Collection中国科学院化学研究所
Corresponding AuthorCao, AM
Affiliation1.Chinese Acad Sci, Shanghai Inst Organ Chem, Polymer Mat Lab, Shanghai 200030, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Organ Chem, Modern Synth Chem Lab, Shanghai 200030, Peoples R China
Recommended Citation
GB/T 7714
Jing, Y,Tian, WS,Li, QB,et al. Novel biodegradable aliphatic poly(butylene succinate-co-cyclic carbonate)s bearing functionalizable carbonate building blocks: II. Enzymatic biodegradation and in vitro biocompatibility assay[J]. BIOMACROMOLECULES,2004,5(6):2258-2268.
APA Jing, Y,Tian, WS,Li, QB,Yang, L,&Cao, AM.(2004).Novel biodegradable aliphatic poly(butylene succinate-co-cyclic carbonate)s bearing functionalizable carbonate building blocks: II. Enzymatic biodegradation and in vitro biocompatibility assay.BIOMACROMOLECULES,5(6),2258-2268.
MLA Jing, Y,et al."Novel biodegradable aliphatic poly(butylene succinate-co-cyclic carbonate)s bearing functionalizable carbonate building blocks: II. Enzymatic biodegradation and in vitro biocompatibility assay".BIOMACROMOLECULES 5.6(2004):2258-2268.
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