ICCAS OpenIR
Analysis of immunomodulating nitric oxide, iNOS and cytokines mRNA in mouse macrophages induced by microcystin-LR
Chen, T; Zhao, XY; Liu, Y; Shi, Q; Hua, ZC; Shen, PP
2004-04-01
Source PublicationTOXICOLOGY
ISSN0300-483X
Volume197Issue:1Pages:67-77
AbstractMicrocystins (MCs) are the toxic molecules produced by common cyanobacterium in freshwater blooms. Their toxicities have brought severe health issues to livestock and human being. Microcystin-LR (MC-LR) is one of the most toxic MCs. This paper presents the profile of the immunomodulation of MC-LR to BALB/c mice peritoneal macrophages. Macrophages were stimulated with 100 mug/l lipopolysaccharide, (LPS) and MC-LR at dose of 1, 10, 100, 1000 nmol/l, respectively, for 24 h. Nitric oxide (NO) production in cell culture supernatants was quantified by using Griess reagent method. Total RNA was extracted from incubated macrophages then the mRNA abundance of induced nitric oxide synthase (iNOS), IL-1beta, TNF-alpha, GM-CSF, IFN-gamma was monitored by using reverse-transcriptional polymerase chain reaction (RT-PCR). The results demonstrated that NO production, mRNA levels of iNOS, IL-1beta, TNF-alpha were down regulated by MC-LR dose-dependently and mRNA levels of GM-CSF and IFN-gamma were also decreased but in dose-independent manner. Our results illustrated the involvement of NO production, iNOS and some cytokines in mice immune system in microcystin shock. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
KeywordMc-lr Macrophage No Inos Cytokine Rt-pcr
DOI10.1016/j.tox.2003.12.013
Indexed BySCI
Language英语
WOS IDWOS:000220325000007
PublisherELSEVIER SCI IRELAND LTD
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81540
Collection中国科学院化学研究所
Corresponding AuthorShen, PP
Affiliation1.Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Dept Biochem, Nanjing 210093, Peoples R China
2.Chinese Acad Sci, Inst Chem, State Key Struct Chem Unstable & Stable Species, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Chen, T,Zhao, XY,Liu, Y,et al. Analysis of immunomodulating nitric oxide, iNOS and cytokines mRNA in mouse macrophages induced by microcystin-LR[J]. TOXICOLOGY,2004,197(1):67-77.
APA Chen, T,Zhao, XY,Liu, Y,Shi, Q,Hua, ZC,&Shen, PP.(2004).Analysis of immunomodulating nitric oxide, iNOS and cytokines mRNA in mouse macrophages induced by microcystin-LR.TOXICOLOGY,197(1),67-77.
MLA Chen, T,et al."Analysis of immunomodulating nitric oxide, iNOS and cytokines mRNA in mouse macrophages induced by microcystin-LR".TOXICOLOGY 197.1(2004):67-77.
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