ICCAS OpenIR
Novel sulfamethazine ligand used for one-step purification of immunoglobulin G from human plasma
Liu, Y; Zhao, R; Shangguan, DH; Zhang, HW; Liu, GQ
2003-07-25
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume792Issue:2Pages:177-185
AbstractTo replace conventional affinity ligand like protein A or protein G, a pseudobioaffinity ligand seems to be an alternative for the purification of immunoglobulin G (IgG). In this study, sulfamethazine (SMZ) was chosen as novel affinity ligand for investigating its affinity to human IgG. Monodisperse, non-porous, cross-linked poly (glycidyl methacrylate) (PGMA) beads were employed as the support for high-performance affinity chromatography. SMZ was immobilized on PGMA beads using bisoxirane (ethanediol diglycigyl ether) as spacer. The resultant affinity media presented minimal non-specific interaction with other proteins. Results of high-performance frontal analysis indicated that the media showed specific affinity to human IgG with a dissociation constant on the order of 10(-6) M. The SMZ affinity column proved useful for a very convenient one-step purification of IgG from human plasma. Antibody purity after a one-step purification was higher than 90%, as determined by densitometric scanninng of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified fraction under reducing condition. The results obtained indicate that SMZ is a valuable affinity ligand for purification of human IgG. (C) 2003 Elsevier B.V. All rights reserved.
KeywordInummoglobulins Sulfamethazine
DOI10.1016/S1570-0232(03)00263-0
Indexed BySCI
Language英语
WOS IDWOS:000184235000004
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81496
Collection中国科学院化学研究所
Corresponding AuthorLiu, GQ
AffiliationChinese Acad Sci, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Liu, Y,Zhao, R,Shangguan, DH,et al. Novel sulfamethazine ligand used for one-step purification of immunoglobulin G from human plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2003,792(2):177-185.
APA Liu, Y,Zhao, R,Shangguan, DH,Zhang, HW,&Liu, GQ.(2003).Novel sulfamethazine ligand used for one-step purification of immunoglobulin G from human plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,792(2),177-185.
MLA Liu, Y,et al."Novel sulfamethazine ligand used for one-step purification of immunoglobulin G from human plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 792.2(2003):177-185.
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