ICCAS OpenIR
Direct chemiluminescence determination of cysteine in human serum using quinine-Ce(IV) system
Nie, LH; Ma, HM; Sun, M; Li, XH; Su, MH; Liang, SC
2003-04-10
Source PublicationTALANTA
ISSN0039-9140
Volume59Issue:5Pages:959-964
AbstractA simple, sensitive and selective chemiluminescence (CL) method was developed for the determination of cysteine. This method is based on that the weak CL of cysteine oxidized with cerium (IV) can be greatly enhanced by quinine. The calibration curve was linear over the range 3.5 x 10(-9)-3.5 x 10(-6) M with a detection limit of 2.5 x 10(-9) M (S/N N = 3). The RSD was found to be 8.4% by 10 replicate determinations of 2.9 x 10(-8) M cysteine. Due to high sensitivity, the proposed method can be used directly to determine the total concentration of cysteine in human serum through simply diluting the sample for a thousand fold. The obtained result was in agreement with that given by amino acid autoanalyzer. The present method does not require any separation, showing a simpler analytical characteristic. The mechanism of the CL reaction was also discussed. (C) 2003 Elsevier Science B.V. All rights reserved.
KeywordChemiluminescence Cysteine Quinine-ce(Iv) System Human Serum
DOI10.1016/S0039-9140(02)00649-5
Indexed BySCI
Language英语
WOS IDWOS:000182494500013
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81484
Collection中国科学院化学研究所
Corresponding AuthorMa, HM
AffiliationChinese Acad Sci, Ctr Mol Sci, Biol Chem Lab, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Nie, LH,Ma, HM,Sun, M,et al. Direct chemiluminescence determination of cysteine in human serum using quinine-Ce(IV) system[J]. TALANTA,2003,59(5):959-964.
APA Nie, LH,Ma, HM,Sun, M,Li, XH,Su, MH,&Liang, SC.(2003).Direct chemiluminescence determination of cysteine in human serum using quinine-Ce(IV) system.TALANTA,59(5),959-964.
MLA Nie, LH,et al."Direct chemiluminescence determination of cysteine in human serum using quinine-Ce(IV) system".TALANTA 59.5(2003):959-964.
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