ICCAS OpenIR
Real time kinetic analysis of the interaction between immunoglobulin G and histidine using quartz crystal microbalance biosensor in solution
Liu, Y; Yu, X; Zhao, R; Shangguan, DH; Bo, ZY; Liu, GQ
2003-10-01
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume18Issue:11Pages:1419-1427
AbstractQuartz crystal microbalance (QCM) biosensor integrated in a flow injection analysis (FIA) system was used for the investigation of the specific interaction between immunoglobin G (IgG) and histidine. The histidine was immobilized on the gold electrodes of the piezoelectric crystal using appropriate procedures based on self-assembling of the dithiothreitol (DTT). The specific interaction of the immobilized ligand with IgG in solution was followed as a change in the resonant frequency of the modified crystal and studied in real time without any additional labels. With the mass sensitive biosensor system, the differences in affinity of three different species of IgG: human IgG, goat IgG and mouse IgG were easily distinguished and their respective kinetic rate constants (k(ass) and k(diss)) and equilibrium association constants (K-A) were determined from the curves of frequency versus time. For the interactions, K-A were 2.92 x 10(4), 3.23 x 10(4) and 4.08 x 10(4) M-1 for human IgG, goat IgG and mouse IgG, respectively. (C) 2003 Elsevier Science B.V. All rights reserved.
KeywordPiezoelectric Biosensor Flow Injection Analysis Kinetic Affinity Constant Immunoglobulin g Histidine
DOI10.1016/S0956-5663(03)00090-3
Indexed BySCI
Language英语
WOS IDWOS:000185193500014
PublisherELSEVIER ADVANCED TECHNOLOGY
Citation statistics
Cited Times:49[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81398
Collection中国科学院化学研究所
Corresponding AuthorLiu, GQ
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Liu, Y,Yu, X,Zhao, R,et al. Real time kinetic analysis of the interaction between immunoglobulin G and histidine using quartz crystal microbalance biosensor in solution[J]. BIOSENSORS & BIOELECTRONICS,2003,18(11):1419-1427.
APA Liu, Y,Yu, X,Zhao, R,Shangguan, DH,Bo, ZY,&Liu, GQ.(2003).Real time kinetic analysis of the interaction between immunoglobulin G and histidine using quartz crystal microbalance biosensor in solution.BIOSENSORS & BIOELECTRONICS,18(11),1419-1427.
MLA Liu, Y,et al."Real time kinetic analysis of the interaction between immunoglobulin G and histidine using quartz crystal microbalance biosensor in solution".BIOSENSORS & BIOELECTRONICS 18.11(2003):1419-1427.
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