ICCAS OpenIR
Dynamic and morphological investigation of phospholipid monolayer hydrolysis by phospholipase C
He, Q; Li, JB
2003-01-10
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume300Issue:2Pages:541-545
AbstractThe hydrolytic reaction of L-alpha-dipalmitoylphosphatidylcholine (L-DPPC) monolayer catalyzed by phospholipase C (PLC) has been investigated using monomolecular membrane technique and Brewster angle microscopy (BAM) at the air/water interface. The curves of surface pressure as a function of time determined a lag-burst process of L-DPPC monolayer hydrolysis by PLC. The BAM images recorded the changes of domains formed in the coexistence region of liquid-condensed (LC) and liquid-expanded (LE) phases during the monolayer hydrolysis. The changes of domain shape and size and the increase of domain number reflect the decrease of reactant and molecular rearrangement process of the main hydrolysis product, dipalmitoylglycerol (DPG). A new phase was observed after the hydrolysis reaction was completed. (C) 2002 Elsevier Science (USA). All rights reserved.
KeywordBrewster Angle Microscopy Hydrolysis Phospholipase c Phospholipid Monolayer
DOI10.1016/S0006-291X(02)02893-0
Indexed BySCI
Language英语
WOS IDWOS:000180607000043
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81392
Collection中国科学院化学研究所
Corresponding AuthorLi, JB
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Int Joint Lab, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
He, Q,Li, JB. Dynamic and morphological investigation of phospholipid monolayer hydrolysis by phospholipase C[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,300(2):541-545.
APA He, Q,&Li, JB.(2003).Dynamic and morphological investigation of phospholipid monolayer hydrolysis by phospholipase C.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,300(2),541-545.
MLA He, Q,et al."Dynamic and morphological investigation of phospholipid monolayer hydrolysis by phospholipase C".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 300.2(2003):541-545.
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