ICCAS OpenIR
Critical factors for reproducible capillary electrophoresis of Escherichia coli cells
Dai, DS; Chen, Y; Qi, L; Yu, X
2003-09-01
Source PublicationELECTROPHORESIS
ISSN0173-0835
Volume24Issue:18Pages:3219-3223
AbstractSharp peaks of Escherichia coli JM 109 (up to 1300 000 theoretical plates) were recorded with either extremely diluted (< 5 mm) or extremely concentrated (ca. 150 mm) Tris-borate (TB) running buffers. However, under the conditions of yielding sharp peaks, migration time of E coli was irreproducible. Critial factors influencing reproducibility were found to include bacterial growth phase, storage condition, cell pretreatment before injection, and concentration of running buffer. Buffer concentrations in the range of 20-100 mm TB were essential for reproducibility. E coli JM 109 was shown to be sensitive to ultrasonification. Bacterial growth and storage conditions could be monitored by CE, with results comparable to those obtained with optical methods.
KeywordCapillary Electrophoresis Escherichia Coli Reproducibility
DOI10.1002/elps.200305600
Indexed ByISTP ; SCI
Language英语
WOS IDWOS:000185897100018
PublisherWILEY-V C H VERLAG GMBH
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81202
Collection中国科学院化学研究所
Corresponding AuthorChen, Y
Affiliation1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Dai, DS,Chen, Y,Qi, L,et al. Critical factors for reproducible capillary electrophoresis of Escherichia coli cells[J]. ELECTROPHORESIS,2003,24(18):3219-3223.
APA Dai, DS,Chen, Y,Qi, L,&Yu, X.(2003).Critical factors for reproducible capillary electrophoresis of Escherichia coli cells.ELECTROPHORESIS,24(18),3219-3223.
MLA Dai, DS,et al."Critical factors for reproducible capillary electrophoresis of Escherichia coli cells".ELECTROPHORESIS 24.18(2003):3219-3223.
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