ICCAS OpenIR
Direct observations of the cleavage-reaction of an L-DPPC monolayer catalyzed by phospholipase A(2) and inhibited by an indole inhibitor at the air/water interface
Zhai, XH; Li, JB; Brezesinski, G; He, Q; Mohwald, H; Lai, LH; Liu, Y; Liu, L; Gao, Y
2003-04-04
Source PublicationCHEMBIOCHEM
ISSN1439-4227
Volume4Issue:4Pages:299-305
AbstractThe enzymatic hydrolysis of an L-dipalmitoylphoisphatidylcholine (L-DPPC) monolayer at the air/water interface, catalyzed by phospholipase A(2) (PLA(2)), serves as a model for biospecific interfacial reactions. The cleavage of L-DPPC was investigated by Brewster angle microscopy. Different types of domain defects were observed to form in the coexisting liquid expanded and liquid condensed phases during the hydrolysis reaction. The adsorption of the enzyme was quantitatively recorded as the increase of the surface pressure over a fixed molecular area with time. In the case of the following processes: adsorption of PLA(2), cleavage reaction, and rearrangement of substrate and product molecules at the interface. Addition of a PLA(2) inhibitor to the lipid monolayer leads to a fast surface pressure increase after enzyme injection. The surface pressure reaches a maximum value and then does not change for a long time. During this period, no change in the domain shape and number density was observed, which indicates that the enzyme is inhibited for a certain period of time. The experimental results provide the possibility of a direct way to prove inhibitor activity.
KeywordBrewster Angle Microscopy Enzymes Hydrolysis Inhibitors Phospholipids
Indexed BySCI
Language英语
WOS IDWOS:000182220400007
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/81170
Collection中国科学院化学研究所
Corresponding AuthorLi, JB
Affiliation1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Int Joint Lab, Beijing 100080, Peoples R China
2.Max Planck Inst Colloids & Interfaces, D-14476 Golm Potsdam, Germany
3.Peking Univ, Inst Phys Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhai, XH,Li, JB,Brezesinski, G,et al. Direct observations of the cleavage-reaction of an L-DPPC monolayer catalyzed by phospholipase A(2) and inhibited by an indole inhibitor at the air/water interface[J]. CHEMBIOCHEM,2003,4(4):299-305.
APA Zhai, XH.,Li, JB.,Brezesinski, G.,He, Q.,Mohwald, H.,...&Gao, Y.(2003).Direct observations of the cleavage-reaction of an L-DPPC monolayer catalyzed by phospholipase A(2) and inhibited by an indole inhibitor at the air/water interface.CHEMBIOCHEM,4(4),299-305.
MLA Zhai, XH,et al."Direct observations of the cleavage-reaction of an L-DPPC monolayer catalyzed by phospholipase A(2) and inhibited by an indole inhibitor at the air/water interface".CHEMBIOCHEM 4.4(2003):299-305.
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