ICCAS OpenIR
Artificial chaperone-assisted renaturation of R-PE subunits
Xie, J; Li, DH; Zhang, JP; Zhao, JQ
2003
Source PublicationNEW JOURNAL OF CHEMISTRY
ISSN1144-0546
Volume27Issue:2Pages:395-398
AbstractThe isolated alpha, beta and two gamma subunits of the R-phycoerythrin (R-PE) from Polysiphonia urceolata were renaturated by the artificial chaperone technique in combination with beta-cyclodextrins. The molecular weights determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ( MALDI-TOF-MS) are 17 580, 19 050, 32 500 and 34 060 for the alpha, beta, gamma(1) and gamma(2) subunits, respectively. The renaturation yields of the four subunits obtained by the artificial chaperone technique are several times higher than those achieved by conventional methods. The two gamma subunits are suggested to be naturally present species, instead of artificial products, considering the molecular weights and spectral features. The existence of two kinds of gamma subunits may explain the failure to determine the entire structures of R-PE, b- or B-phycoerythrin (b- or B-PE) by X-ray crystal structural analyses.
DOI10.1039/b209089a
Indexed BySCI
Language英语
WOS IDWOS:000180515200043
PublisherROYAL SOC CHEMISTRY
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/80978
Collection中国科学院化学研究所
Corresponding AuthorZhao, JQ
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Xie, J,Li, DH,Zhang, JP,et al. Artificial chaperone-assisted renaturation of R-PE subunits[J]. NEW JOURNAL OF CHEMISTRY,2003,27(2):395-398.
APA Xie, J,Li, DH,Zhang, JP,&Zhao, JQ.(2003).Artificial chaperone-assisted renaturation of R-PE subunits.NEW JOURNAL OF CHEMISTRY,27(2),395-398.
MLA Xie, J,et al."Artificial chaperone-assisted renaturation of R-PE subunits".NEW JOURNAL OF CHEMISTRY 27.2(2003):395-398.
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