ICCAS OpenIR
Single-mismatch detection using nucleic acid molecular 'light switch'
Ling, LS; He, ZK; Chen, F; Zeng, YE
2003-02-06
Source PublicationTALANTA
ISSN0039-9140
Volume59Issue:2Pages:269-275
AbstractA novel nucleic acid molecular 'light switch' method is developed for the sensitive recognition and detection of a single-base mismatched oligonucleotides. The detection limit of oligonucleotide of perfect double stranded and that with single-base, two-base and three-base mismatched are 0.11, 0.17, 0.34 and 1.5 ng ml(-1), respectively. It was found that Ru(phen)(2)(d PPX)(2+) (phen = 1,10-phenanthroline, dppx = 7,8-dimethyl-dipyridophenazine) can be used to detect and recognize the perfect double stranded oligonucleotides from mismatched and random targets by the intensity of fluorescence and temperature. This method can be used to recognize and quantitatively detect target DNA with specific sequence. The advantage of this method is that no requisites are needed to separate the coexisting random targets in the case of a mixed solution containing perfect, mismatched and random targets, which make the recognition analysis of oligonucleotide simple and fast. Moreover, it has potential in the study of dynamic process of DNA hybridization. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordMolecular 'light Switch' Fluorimetry Dna Ru(Phen)(2)(Dppx)(2+)
Indexed BySCI
Language英语
WOS IDWOS:000180614100007
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/80896
Collection中国科学院化学研究所
Corresponding AuthorHe, ZK
Affiliation1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Ling, LS,He, ZK,Chen, F,et al. Single-mismatch detection using nucleic acid molecular 'light switch'[J]. TALANTA,2003,59(2):269-275.
APA Ling, LS,He, ZK,Chen, F,&Zeng, YE.(2003).Single-mismatch detection using nucleic acid molecular 'light switch'.TALANTA,59(2),269-275.
MLA Ling, LS,et al."Single-mismatch detection using nucleic acid molecular 'light switch'".TALANTA 59.2(2003):269-275.
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