ICCAS OpenIR
Determination of carbohydrates as their 3-aminophthalhydrazide derivatives by capillary zone electrophoresis with on-line chemiluminescence detection
Wang, XY; Wang, Q; Chen, Y; Han, HW
2003-04-11
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume992Issue:1-2Pages:181-191
AbstractA method based on pre-capillary derivatization with luminol (3-aminophthalhydrazide) for carbohydrate analysis using capillary electrophoresis with on-line chemiluminescence (CL) detection was developed. The derivatives of seven monosaccharides were separated and detected by using 200 mM borate buffer containing 100 mM hydrogen peroxide at pH 10.0 as separation electrolyte and 25 mM hexacyanoferrate in 3 M sodium hydroxide solution as post-capillary chemiluminescence reagent with separation efficiencies ranging from 160,000 to 231,000 plates per metre. The minimum amount of carbohydrate derivatized was 2 pmol (corresponding to the concentration of 2 muM). The method also provided a linear response for glucose in the concentration range of 0.1-250 muM with a mass detection limit of 420 amol or a concentration detection limit of 0.1 muM. Preliminary work using the CE-CL format to determine glucose in a rat brain microdialysis sample is presented as a typical case. (C) 2003 Elsevier Science B.V. All rights reserved.
KeywordDerivatization Electrophoresis Detection Electrophoresis Chemiluminescence Detection Carbohydrates Aminophthalhydrazide Luminol
DOI10.1016/S0021-9673(03)00273-5
Indexed BySCI
Language英语
WOS IDWOS:000182147600018
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/80820
Collection中国科学院化学研究所
Corresponding AuthorWang, XY
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wang, XY,Wang, Q,Chen, Y,et al. Determination of carbohydrates as their 3-aminophthalhydrazide derivatives by capillary zone electrophoresis with on-line chemiluminescence detection[J]. JOURNAL OF CHROMATOGRAPHY A,2003,992(1-2):181-191.
APA Wang, XY,Wang, Q,Chen, Y,&Han, HW.(2003).Determination of carbohydrates as their 3-aminophthalhydrazide derivatives by capillary zone electrophoresis with on-line chemiluminescence detection.JOURNAL OF CHROMATOGRAPHY A,992(1-2),181-191.
MLA Wang, XY,et al."Determination of carbohydrates as their 3-aminophthalhydrazide derivatives by capillary zone electrophoresis with on-line chemiluminescence detection".JOURNAL OF CHROMATOGRAPHY A 992.1-2(2003):181-191.
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