ICCAS OpenIR
Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats
Wang, L; Li, YZ; Han, HW; Liu, GQ; Osborne, PG
2003-06-26
Source PublicationNEUROSCIENCE LETTERS
ISSN0304-3940
Volume344Issue:2Pages:91-94
AbstractO-2 concentration ([O-2]) in air equilibrated solutions at room temperature is three fold higher than that in brain extracellular fluid (ECF), and CO2 concentration ([CO2]) is 100 times lower. Using microdialysis the ECF is routinely dialyzed against glucose free isotonic perfusates containing 200 muM O-2 and 10 muM CO2. In conscious rats, 2 days after probe implantation, decreasing perfusate [O-2] from 200 to 68 muM (physiologic level) for 60 min, while maintaining a low [CO2] (10 muM), increased striatal dialysate glucose and lactate by 12% and 33%, respectively. The same, protocol on the third day essentially had no effect on monoamine metabolites. Decreasing [O-2] concurrent with increasing [CO2] to 1.3 mM (physiologic level) for 60 min increased glucose and lactate by 17% and 37%, respectively. This study demonstrates for dialysis studies of glucose and lactate, perfusates that mimic physiologic ECF [O-2] and [CO2] are more appropriate. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
KeywordPerfusion Hyperoxia Hypoxia Metabolism Glucose Lactate
DOI10.1016/S0304-3940(03)00448-8
Indexed BySCI
Language英语
WOS IDWOS:000183347400006
PublisherELSEVIER SCI IRELAND LTD
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/80808
Collection中国科学院化学研究所
Corresponding AuthorOsborne, PG
Affiliation1.Chinese Acad Sci, Ctr Mol Sci, Inst Chem, Beijing 100080, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Wang, L,Li, YZ,Han, HW,et al. Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats[J]. NEUROSCIENCE LETTERS,2003,344(2):91-94.
APA Wang, L,Li, YZ,Han, HW,Liu, GQ,&Osborne, PG.(2003).Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats.NEUROSCIENCE LETTERS,344(2),91-94.
MLA Wang, L,et al."Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats".NEUROSCIENCE LETTERS 344.2(2003):91-94.
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