ICCAS OpenIR
Determination of stachyose in Stachys Sieboldii Miq. by high performance liquid chromatography with evaporative light scattering detection
Yin, JF; Yang, GL; Li, ZW; Liu, HY; Fan, ZL; Chen, Y
2003-12-01
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume31Issue:12Pages:1409-1412
AbstractA method for the determination of stachyose in Stachys sieboldii miq. by high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) was established. Two different extraction crafts were designed and compared. Stachyose was successfully separated on a Bondapak NH2 column (300 mm x 3.9 mm i.d.) at ambient temperature, the mobile phase composition was a binary solvent mixture of methanol-water (80:20, V/V). The flow rate was 1.0 mL/min. The parameters of ELSD were set as follows: drift tube temperature was 40degreesC, nitrogen carrier gas flow was 4.0 L/min. This method fulfilled all the standard requirements of linearity, accuracy and precision. The linear range was 1.02similar to12.22 mug (r=0.9995). The detection limit was 0.10 mug and the average recovery was 91.6% (RSD=1.43%). The contents of stachyose by two different extraction crafts were 29.8% and 25.2%, the purity were 63.76% and 88.35%, respectively. This method was accurate, rapid, simple and reproducible, and suitable for the integrated analysis of stachyose.
KeywordHigh Performance Liquid Chromatography Evaporative Light Scattering Detection Stachyose Stachys Sieboldii Miq
Indexed BySCI
Language英语
WOS IDWOS:000188504100001
PublisherSCIENCE CHINA PRESS
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/80684
Collection中国科学院化学研究所
Corresponding AuthorYang, GL
Affiliation1.Coll Chem & Environm Sci, Baoding 071002, Peoples R China
2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yin, JF,Yang, GL,Li, ZW,et al. Determination of stachyose in Stachys Sieboldii Miq. by high performance liquid chromatography with evaporative light scattering detection[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2003,31(12):1409-1412.
APA Yin, JF,Yang, GL,Li, ZW,Liu, HY,Fan, ZL,&Chen, Y.(2003).Determination of stachyose in Stachys Sieboldii Miq. by high performance liquid chromatography with evaporative light scattering detection.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,31(12),1409-1412.
MLA Yin, JF,et al."Determination of stachyose in Stachys Sieboldii Miq. by high performance liquid chromatography with evaporative light scattering detection".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 31.12(2003):1409-1412.
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