ICCAS OpenIR
Improved method for the routine determination of acetylcholine and choline in brain microdialysate using a horseradish peroxidase column as the immobilized enzyme reactor
Dong, Y; Wang, L; Shangguan, DH; Zhao, R; Liu, GQ
2003-05-05
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume788Issue:1Pages:193-198
AbstractA modified microbore high-performance liquid chromatography-immobilized enzyme reactor-electrochemical detection system for acetylcholine (ACh) and choline (Ch) was developed. The system used the horseradish peroxidase and a solution mediator ferrocene to convert the analyte into an oxidized ferrocene species which was detected electrochemically by reduction at 0 mV. There was an excellent linear relationship between the concentration of ACh/Ch and the peak height over the range of 1-5000 nmol/l. The limit of detection for ACh was 2 fmol/5 mul (S/N=3:1). Compared with the common method recommended by Bioanalytical System Inc. (BAS), this method exhibits a 200-fold improvement in the detection limit. The ACh and Ch levels in rat brain microdialysate were examined. (C) 2003 Elsevier Science B.V. All rights reserved.
KeywordMicrodialysis Acetylcholine Choline Horseradish Peroxidase
DOI10.1016/S1570-0232(02)01008-5
Indexed BySCI
Language英语
WOS IDWOS:000182059400021
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/80608
Collection中国科学院化学研究所
Corresponding AuthorShangguan, DH
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Dong, Y,Wang, L,Shangguan, DH,et al. Improved method for the routine determination of acetylcholine and choline in brain microdialysate using a horseradish peroxidase column as the immobilized enzyme reactor[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2003,788(1):193-198.
APA Dong, Y,Wang, L,Shangguan, DH,Zhao, R,&Liu, GQ.(2003).Improved method for the routine determination of acetylcholine and choline in brain microdialysate using a horseradish peroxidase column as the immobilized enzyme reactor.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,788(1),193-198.
MLA Dong, Y,et al."Improved method for the routine determination of acetylcholine and choline in brain microdialysate using a horseradish peroxidase column as the immobilized enzyme reactor".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 788.1(2003):193-198.
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