Screening of affinity peptide ligands from combinatorial peptide libraries
Fang, CL; Zhao, R; Liu, Y; Yu, X; Xiong, SX; Liu, GQ
AbstractA new method for the rapid and efficient screening of affinity ligands to biological targets is reported. The fusion peptide of influenza virus A was used as the model target and immobilized on the PGMA beads. Antisense peptide YRSKQA of fusion peptide was chosen as the lead compound. The special positional scanning peptide libraries were designed based on YRSKQA and synthesized by utilizing solid phase peptide synthesis manually. The libraries were YRSKQX, YRSKXA, YRSXQA, YRXKQA, YXSKQA and XRSKQA, where X represented 18 L-amino acids(except for Cys and Trp). Each library was screened by affinity chromatography. The eluates from the fusion peptide affinity column were collected and analyzed by RP-HPLC and MS, respectively, in order to determine the kind of X at each position. After the preferred residues of six positions were decided, the two preferred peptide sequences, GRGKHK and TRGKHK, were obtained. The dissociation constants of GRGKHK, TRGKHK and YRSKQA, were 3.35 X 10(-6), 5.24 X 10(-6) and 1.15 X 10(-5) mol (.) L-1, respectively. The preferred peptides showed the higher affinity binding to immobilized fusion peptide than the lead peptide.
KeywordCombinatorial Peptide Libraries Affinity Ligands Dissociation Constant Solid Phase Peptide Synthesis
Indexed BySCI
WOS IDWOS:000182666400016
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorZhao, R
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Fang, CL,Zhao, R,Liu, Y,et al. Screening of affinity peptide ligands from combinatorial peptide libraries[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2003,24(1):52-54.
APA Fang, CL,Zhao, R,Liu, Y,Yu, X,Xiong, SX,&Liu, GQ.(2003).Screening of affinity peptide ligands from combinatorial peptide libraries.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,24(1),52-54.
MLA Fang, CL,et al."Screening of affinity peptide ligands from combinatorial peptide libraries".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 24.1(2003):52-54.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Fang, CL]'s Articles
[Zhao, R]'s Articles
[Liu, Y]'s Articles
Baidu academic
Similar articles in Baidu academic
[Fang, CL]'s Articles
[Zhao, R]'s Articles
[Liu, Y]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Fang, CL]'s Articles
[Zhao, R]'s Articles
[Liu, Y]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.