ICCAS OpenIR
Screening of affinity peptide ligands from combinatorial peptide libraries
Fang, CL; Zhao, R; Liu, Y; Yu, X; Xiong, SX; Liu, GQ
2003
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN0251-0790
Volume24Issue:1Pages:52-54
AbstractA new method for the rapid and efficient screening of affinity ligands to biological targets is reported. The fusion peptide of influenza virus A was used as the model target and immobilized on the PGMA beads. Antisense peptide YRSKQA of fusion peptide was chosen as the lead compound. The special positional scanning peptide libraries were designed based on YRSKQA and synthesized by utilizing solid phase peptide synthesis manually. The libraries were YRSKQX, YRSKXA, YRSXQA, YRXKQA, YXSKQA and XRSKQA, where X represented 18 L-amino acids(except for Cys and Trp). Each library was screened by affinity chromatography. The eluates from the fusion peptide affinity column were collected and analyzed by RP-HPLC and MS, respectively, in order to determine the kind of X at each position. After the preferred residues of six positions were decided, the two preferred peptide sequences, GRGKHK and TRGKHK, were obtained. The dissociation constants of GRGKHK, TRGKHK and YRSKQA, were 3.35 X 10(-6), 5.24 X 10(-6) and 1.15 X 10(-5) mol (.) L-1, respectively. The preferred peptides showed the higher affinity binding to immobilized fusion peptide than the lead peptide.
KeywordCombinatorial Peptide Libraries Affinity Ligands Dissociation Constant Solid Phase Peptide Synthesis
Indexed BySCI
Language英语
WOS IDWOS:000182666400016
PublisherHIGHER EDUCATION PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/80372
Collection中国科学院化学研究所
Corresponding AuthorZhao, R
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Fang, CL,Zhao, R,Liu, Y,et al. Screening of affinity peptide ligands from combinatorial peptide libraries[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2003,24(1):52-54.
APA Fang, CL,Zhao, R,Liu, Y,Yu, X,Xiong, SX,&Liu, GQ.(2003).Screening of affinity peptide ligands from combinatorial peptide libraries.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,24(1),52-54.
MLA Fang, CL,et al."Screening of affinity peptide ligands from combinatorial peptide libraries".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 24.1(2003):52-54.
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