ICCAS OpenIR
Specific aptamer-protein interaction studied by atomic force microscopy
Jiang, YX; Zhu, CF; Ling, LS; Wan, LJ; Fang, XH; Bai, C
2003-05-01
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume75Issue:9Pages:2112-2116
AbstractAptamers are a new class of synthetic DNA/RNA oligo-nucleotides generated from in vitro selection to selectively bind with various molecules. Due to their molecular recognition capability for proteins, aptamers are becoming promising reagents in protein detection and new drug development. In this study, the specific interaction between the protein immunoglobulin E (IgE) and its 37-nt aptamer has been measured directly by atomic force microscopy. The single-molecule unbinding force between IgE and the aptamer is determined using the Poisson statistical method. The individual unbinding force between IgE and its monoclonal antibody has also been obtained and compared to that between IgE and the aptamer. The results reveal the high affinity of the aptamer to protein, which could match or even surpass that of the antibody to its antigen.
DOI10.1021/ac026182s
Indexed BySCI
Language英语
WOS IDWOS:000182662800034
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:106[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/80278
Collection中国科学院化学研究所
Corresponding AuthorFang, XH
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Jiang, YX,Zhu, CF,Ling, LS,et al. Specific aptamer-protein interaction studied by atomic force microscopy[J]. ANALYTICAL CHEMISTRY,2003,75(9):2112-2116.
APA Jiang, YX,Zhu, CF,Ling, LS,Wan, LJ,Fang, XH,&Bai, C.(2003).Specific aptamer-protein interaction studied by atomic force microscopy.ANALYTICAL CHEMISTRY,75(9),2112-2116.
MLA Jiang, YX,et al."Specific aptamer-protein interaction studied by atomic force microscopy".ANALYTICAL CHEMISTRY 75.9(2003):2112-2116.
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