ICCAS OpenIR
Quartz crystal biosensor for real-time monitoring of molecular recognition between protein and small molecular medicinal agents
Liu, Y; Yu, X; Zhao, R; Shangguan, DH; Bo, ZY; Liu, GQ
2003-10-30
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume19Issue:1Pages:9-19
AbstractA quartz crystal microbalance (QCM) biosensor integrated into a flow injection analysis (FIA) system was used for the real-time investigation of molecular recognition between a protein and small molecular medicinal agents. Two sulfa-drugs, sulfamethazine (SMZ) and sulfamethoxazole (SMO), were, respectively, immobilized on the gold electrodes of the piezoelectric crystals using appropriate procedures based on self-assembly of the dithiothreitol (DTT). The binding interactions of the two immobilized drug ligands, with various proteins in solution, were followed as changes in the resonant frequency of the modified crystals. Results obtained from this rapid screen analysis clearly indicated that the two drug ligands appeared quite different in this molecular recognition procedure although their structures were similar. SMZ-immobilized sensor showed specific interaction only with IgG, while SMO-imobilized sensor showed negligible specific binding with IgG, but binding with trypsin and chymotrypsin. Further studies oil the specific interaction between immobilized SMZ and three different species of IgG-human IgG, goat IgG and mouse IgG were carried Out and the marked species-dependent difference was observed. The resultant sensorgrams were rapidly analyzed by using an in-house kinetic analysis software based on genetic algorithm (GA) to derive both the kinetic rate constants (k(ass) and k(diss)) and equilibrium association constants (K-A) for IgG-SMZ interactions. For the interactions, K-A were 5.48 x 10(5), 2.75 X 10(5) and 1.86 X 10(5) M-1 for human IgG, goat IgG and mouse IgG, respectively. The kinetic data provided further insight into the structural/ functional relationships of different IgG on a molecular level. (C) 2003 Elsevier B.V. All rights reserved.
KeywordQuartz Crystal Biosensor Kinetic Analysis Molecular Recognition Immunoglobulin g Sulfamethazine Sulfamethoxazole Genetic Algorithm
DOI10.1016/S0956-5663(03)00127-1
Indexed BySCI
Language英语
WOS IDWOS:000186242000002
PublisherELSEVIER ADVANCED TECHNOLOGY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/80220
Collection中国科学院化学研究所
Corresponding AuthorLiu, GQ
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Liu, Y,Yu, X,Zhao, R,et al. Quartz crystal biosensor for real-time monitoring of molecular recognition between protein and small molecular medicinal agents[J]. BIOSENSORS & BIOELECTRONICS,2003,19(1):9-19.
APA Liu, Y,Yu, X,Zhao, R,Shangguan, DH,Bo, ZY,&Liu, GQ.(2003).Quartz crystal biosensor for real-time monitoring of molecular recognition between protein and small molecular medicinal agents.BIOSENSORS & BIOELECTRONICS,19(1),9-19.
MLA Liu, Y,et al."Quartz crystal biosensor for real-time monitoring of molecular recognition between protein and small molecular medicinal agents".BIOSENSORS & BIOELECTRONICS 19.1(2003):9-19.
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