ICCAS OpenIR
Adsorption of glucose oxidase into lipid monolayers: effect of a lipid headgroup charge
Li, JR; Rosilio, V; Boissonnade, MM; Baszkin, A
2003-05-15
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume29Issue:1Pages:13-20
AbstractThe effect of both lipid headgroup charge and of aqueous subphase pH on adsorption of glucose oxidase molecules (GOx) into dibehenoylphosphatidylcholine (DBPC) and octadecylamine (ODA) monolayers has been studied by surface tension and surface pressure measurements. The results revealed that whereas the extent of GOx adsorption into pure DBPC monolayers essentially depended on the area in a monolayer available to harbor adsorbing GOx molecules, enhanced enzyme penetration into a monolayer occurred at high surface densities. The kinetics of enzyme penetration was found to be dependent of the initial surface pressure of a spread monolayer. The addition of ODA to DBPC monolayers gave a dramatic boost to GOx penetration and at high molecular surface densities largely depended on the ratio of ODA in the mixed monolayers. This increase in GOx penetration was attributed to the strong attractive electrostatic interaction between the positively charged ODA and the negatively charged enzyme. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordProtein Adsorption Surface Tension Enzyme Glucose Oxidase Phospholipid Gox Penetration
Indexed BySCI
Language英语
WOS IDWOS:000182777100002
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/80048
Collection中国科学院化学研究所
Corresponding AuthorRosilio, V
Affiliation1.Lab Physicochim Surface, CNRS, UMR 8612, F-92296 Chatenay Malabry, France
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Li, JR,Rosilio, V,Boissonnade, MM,et al. Adsorption of glucose oxidase into lipid monolayers: effect of a lipid headgroup charge[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2003,29(1):13-20.
APA Li, JR,Rosilio, V,Boissonnade, MM,&Baszkin, A.(2003).Adsorption of glucose oxidase into lipid monolayers: effect of a lipid headgroup charge.COLLOIDS AND SURFACES B-BIOINTERFACES,29(1),13-20.
MLA Li, JR,et al."Adsorption of glucose oxidase into lipid monolayers: effect of a lipid headgroup charge".COLLOIDS AND SURFACES B-BIOINTERFACES 29.1(2003):13-20.
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