ICCAS OpenIR
Plasma-treated, collagen-anchored polylactone: Its cell affinity evaluation under shear or shear-free conditions
Yang, J; Wan, YQ; Yang, JL; Bei, JZ; Wang, SG
2003-12-15
Source PublicationJOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
ISSN0021-9304
Volume67AIssue:4Pages:1139-1147
AbstractPoly(L-lactic acid)(PLLA) and poly(L-lactic-co-glycolic acid) (PLGA) (85/15) were modified by plasma treatment. Then they were collagen anchored (PT/CA), and the cell affinity was evaluated by cell culture under shear or shear-free conditions. A convenient and "intuitionistic" dyeing method has been proposed for measuring the modified depth when plasma treatment is applied for the treatment of porous scaffolds. A parallel plate flow chamber was developed in order to study the cell affinity of a material under shear stress. Our results show that a porous scaffold can be modified by plasma treatment and that a depth of about 4.0 mm for this modification can be reached with NH3 plasma treatment (50 w, 20 Pa, 5 min). PT/CA modification is an effective surface modification method for facilitating cell transplantation and improving the cell affinity of three-dimensional porous cell scaffolds in tissue engineering. It can solve the problem of non-uniform cell distribution in most synthetic porous cell scaffolds. Using the flow chamber system, a series of quantitative data, including cell adherent fraction, cell area, and mean shape, were compared to evaluate the cell affinity of PLLA before and after PT/CA modification. The results indicate that the quality of cell attachment on PT/CA-modified PLLA apparently is better than that on unmodified PLLA. The flow chamber system potentially may be a tool for evaluating surface modification methods. (C) 2003 Wiley Periodicals, Inc.
KeywordPolylactone Surface Modification Flow Chamber Cell Affinity Tissue Engineering
DOI10.1002/jbm.a.10034
Indexed BySCI
Language英语
WOS IDWOS:000186877700009
PublisherWILEY-LISS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79750
Collection中国科学院化学研究所
Corresponding AuthorWang, SG
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yang, J,Wan, YQ,Yang, JL,et al. Plasma-treated, collagen-anchored polylactone: Its cell affinity evaluation under shear or shear-free conditions[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2003,67A(4):1139-1147.
APA Yang, J,Wan, YQ,Yang, JL,Bei, JZ,&Wang, SG.(2003).Plasma-treated, collagen-anchored polylactone: Its cell affinity evaluation under shear or shear-free conditions.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,67A(4),1139-1147.
MLA Yang, J,et al."Plasma-treated, collagen-anchored polylactone: Its cell affinity evaluation under shear or shear-free conditions".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 67A.4(2003):1139-1147.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, J]'s Articles
[Wan, YQ]'s Articles
[Yang, JL]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, J]'s Articles
[Wan, YQ]'s Articles
[Yang, JL]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, J]'s Articles
[Wan, YQ]'s Articles
[Yang, JL]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.