Studies on conducting composite material-glucose oxidase biosensor
Zhang, GL; Pan, XH; Kan, JQ; Zhang, JH; Li, YF
AbstractThis paper reports the preparation and properties of biosenor using conducting composite material consisted of ethyl cellulose and acetylene black. The final biosensor was constructed by applying a mixture containing 0.010 g ethyl cellulose, 0.004 g acetylene black, a little paraffin wax and 0.2 mg glucose oxidase (23000 units/g solid, E. C. 1. 1. 3. 4, Sigma Chemical Co.) on a platinum electrode (3 mm x 3 mm). Effect of various factors on the response current of the biosensor was discussed. The response current increases linearly with increasing glucose concentration under 11.5 mmol . dm(-3). Its activation energy is 40.3 kJ . mol(-1) in enzyme-catalyzed reaction. AFM shows that the surface of conducting polymer composite material-glucose oxidase electrode's presents uniform granular structure after paraffin wax being washed with cyclohexane, which is favorable for enzyme-catalyzed reaction.
KeywordBiosensor Conducting Composite Material Glucose Oxidase Ethyl Cellulose Acetylene Black
Indexed BySCI
WOS IDWOS:000183785400011
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Document Type期刊论文
Corresponding AuthorKan, JQ
Affiliation1.Yangzhou Univ, Sch Sci, Dept Chem, Yanngzhou 225002, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, GL,Pan, XH,Kan, JQ,et al. Studies on conducting composite material-glucose oxidase biosensor[J]. ACTA PHYSICO-CHIMICA SINICA,2003,19(6):533-537.
APA Zhang, GL,Pan, XH,Kan, JQ,Zhang, JH,&Li, YF.(2003).Studies on conducting composite material-glucose oxidase biosensor.ACTA PHYSICO-CHIMICA SINICA,19(6),533-537.
MLA Zhang, GL,et al."Studies on conducting composite material-glucose oxidase biosensor".ACTA PHYSICO-CHIMICA SINICA 19.6(2003):533-537.
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