ICCAS OpenIR
Probing connection of PBS with the photosystems in intact cells of Spirulina platensis by temperature-induced fluorescence fluctuation
Li, DH; Xie, H; Zhao, YW; Zhao, JQ
2003-03-06
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
ISSN0005-2728
Volume1557Issue:1-3Pages:35-40
AbstractTemperature-dependent fluorescence for intact cells of cyanobacterium Spirulina platensis was detected to search for the connection of the phycobilisome (PBS) with Photosystem I (PSI) and Photosystem II (PSII). Some interesting results were obtained from the deconvoluted fluorescence components of C-phycocyanin (C-PC), allophycocyanin (APC), PSI and PSII as well as the fluorescence spectra of the intact cells at room temperature (RT=25degreesC) and 0degreesC. It was observed that, compared to those at RT, both of the fluorescence components for PSI and APC increased, whereas those for PSII and C-PC decreased at 0degreesC with excitation at 580 mn, that is, the fluorescence for C-PC is not synchronous with that for APC, and the fluorescence fluctuation for PSI is not synchronous with that for PSII. On the other hand, the decrease in C-PC fluorescence is synchronous with the increase in PSI fluorescence, and the increase in APC fluorescence is synchronous with the decrease in PSII fluorescence. Therefore, it can be readily deduced that PBS should be coupled not only with PSII through the terminal acceptors in the APC core but also with PSI through C-PC in PBS rods at physiological condition, while at 0degreesC, a migration of a PBS makes the APC partially detached from PSII but the C-PC more efficiently coupled with PSI. The results provide good evidences for "mobile PBS" model and "parallel connection" model but not for the "spillover" model. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordC-phycocyanin Allophycocyanin Photosystem Thylakoid Membrane
DOI10.1016/S0005-2728(02)00392-4
Indexed BySCI
Language英语
WOS IDWOS:000181495600004
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79696
Collection中国科学院化学研究所
Corresponding AuthorZhao, JQ
AffiliationAcad Sinica, Ctr Mol Sci, Inst Chem, Chinese Acad Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Li, DH,Xie, H,Zhao, YW,et al. Probing connection of PBS with the photosystems in intact cells of Spirulina platensis by temperature-induced fluorescence fluctuation[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2003,1557(1-3):35-40.
APA Li, DH,Xie, H,Zhao, YW,&Zhao, JQ.(2003).Probing connection of PBS with the photosystems in intact cells of Spirulina platensis by temperature-induced fluorescence fluctuation.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1557(1-3),35-40.
MLA Li, DH,et al."Probing connection of PBS with the photosystems in intact cells of Spirulina platensis by temperature-induced fluorescence fluctuation".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1557.1-3(2003):35-40.
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