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Study of protein binding of acidic drug, nateglinide, in human plasma albumin solution by high performance capillary electrophoresis/frontal analysis
Zhao, YY; Yang, GL; Fan, ZL; Wang, DX; Chen, Y
2003-07-01
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume31Issue:7Pages:860-864
AbstractThe protein binding constants, binding sites and binding rate of acidic drug, Nateglinide, in human Plasma albumin solution was studied by high performance capillary electrophoresis frontal analysis (HPCE/FA). The experiments were carried out by determining unbound drug concentration in drug-protein equilibrium solution in a uncoated fused silica capillary (40 cm x 50 mum i.d.; effective length 32 cm) for capillary electrophoresis under the following conditions: background solution, sodium phosphate buffer solution (pH 7.4, ionic strength 0.17); ultraviolet detector, wavelength 214 nm; hydrostatic injection mode and the injection time 100 s; run voltage 18 kV. The unbound drug concentration. was calculated from the plateau height of the profile with good linear relation, r > 0.9999 for nateglinide ( n = 6). The influence of different protein concentrations on the protein binding was investigated while the drug concentration (200 mumol/L, 250 mumol/L) was fixed. Similarly, the effect of different drug concentrations on protein binding was also studied while the protein concentration ( 100 mumol/L) was fixed. The experimental data were fitted by a non-linear least-squares curve fitting program and binding parameters were obtained simultaneously. The precision of the data determined by HPCE/FA was RSD (RSD < 2.5% n = 3). The capillary electrophoresis frontal analysis presents an easy, accurate. method for pharmacokinetics and pharmacodynamics study of the drug.
KeywordAcidic Drug Nateglinide Protein Binding Parameter Capillary Electrophoresis/frontal Analysis
Indexed BySCI
Language英语
WOS IDWOS:000184609900023
PublisherSCIENCE CHINA PRESS
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79670
Collection中国科学院化学研究所
Corresponding AuthorZhao, YY
Affiliation1.Hebei Med Coll Continuing Educ, Expt Ctr, Baoding 071000, Peoples R China
2.Hebei Univ, Coll Chem & Environm Sci, Baoding 071002, Peoples R China
3.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhao, YY,Yang, GL,Fan, ZL,et al. Study of protein binding of acidic drug, nateglinide, in human plasma albumin solution by high performance capillary electrophoresis/frontal analysis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2003,31(7):860-864.
APA Zhao, YY,Yang, GL,Fan, ZL,Wang, DX,&Chen, Y.(2003).Study of protein binding of acidic drug, nateglinide, in human plasma albumin solution by high performance capillary electrophoresis/frontal analysis.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,31(7),860-864.
MLA Zhao, YY,et al."Study of protein binding of acidic drug, nateglinide, in human plasma albumin solution by high performance capillary electrophoresis/frontal analysis".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 31.7(2003):860-864.
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