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New triazine spectroscopic reagent for the separation of DL-amino acids by micellar electrokinetic chromatography
Ma, HM; Wang, ZH; Su, MH
2002-04-26
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume955Issue:1Pages:125-131
AbstractAn approach to the chiral separation of racemic mixtures of amino acids by means of micellar electrokinetic chromatography after derivatization with a new triazine spectroscopic reagent, 3-(4,6-dichloro-1,3,5-triazinylamino)-7-dimethylamino-2-methylphenazine (DTDP), has been evaluated. It was found that the derivatives of the aliphatic amino acids such as serine, valine and arginine, could produce a strong UV absorption at 282 nm, whose apparent molar absorptivities are of 10(4) M-1 cm(-1), and thus the concentration of the amino acids down to 3 X 10(-7) M can still give a detectable signal (SIN = 3). beta-Cyclodextrin (beta-CD) added to the buffer system was used as a chiral selector, and separation conditions were optimized. The presence of an organic modifier (2-propanol) was also a prerequisite for the chiral separation. The best results for the chiral separation of DTDP-amino acids were achieved in a mixed sodium dodecylsulfate-beta-CD-borate-2-propanol medium at pH 9.0. Compared to some of the commonly used derivatization methods, the present one offers a relatively stable derivative and strong U-V absorption for the spectroscopically inert amino acids, thus enabling amino acids to be separated and detected by CE even with a simpler UV detector. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordDerivatization Electrophoresis Enantiomer Separation Triazines Amino Acids Dichlorotriazinylamino-di-methylaminomethylphenazine
Indexed BySCI
Language英语
WOS IDWOS:000175433800012
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79620
Collection中国科学院化学研究所
Corresponding AuthorMa, HM
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Ma, HM,Wang, ZH,Su, MH. New triazine spectroscopic reagent for the separation of DL-amino acids by micellar electrokinetic chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2002,955(1):125-131.
APA Ma, HM,Wang, ZH,&Su, MH.(2002).New triazine spectroscopic reagent for the separation of DL-amino acids by micellar electrokinetic chromatography.JOURNAL OF CHROMATOGRAPHY A,955(1),125-131.
MLA Ma, HM,et al."New triazine spectroscopic reagent for the separation of DL-amino acids by micellar electrokinetic chromatography".JOURNAL OF CHROMATOGRAPHY A 955.1(2002):125-131.
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