ICCAS OpenIR
Analysis of carbohydrates by capillary zone electrophoresis with on-capillary derivatization
Wang, XY; Chen, Y; Li, Z; Wang, Z
2002
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
Volume25Issue:4Pages:589-600
AbstractCarbohydrates were on-capillary derivatized by p-hydrazine-benzenesulfonic acid (BHZ) and separated by capillary zone electrophoresis using 100 mM boric acid (pH 10.2) as running buffer. The derivatization was achieved by keeping the capillary tip introduced with sample in between two zones of labeling reagent at room temperature for 30 min. The detection limit of galactose and glucose were 15.6 and 31.2 mum, respectively, a bit higher than the corresponding values from pre-capillary derivatization (10.5 and 3.6 muM, respectively). Nevertheless the sample consumption was decreased by two to three orders of magnitude compared to the pre-capillary mode. Reproducible quantification of carbohydrates was demonstrated in the concentration range of 0.3-30 mM. The application of this method to determine the glucose in human serum was also present.
Indexed BySCI
Language英语
WOS IDWOS:000174424300005
PublisherMARCEL DEKKER INC
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79512
Collection中国科学院化学研究所
Corresponding AuthorChen, Y
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wang, XY,Chen, Y,Li, Z,et al. Analysis of carbohydrates by capillary zone electrophoresis with on-capillary derivatization[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2002,25(4):589-600.
APA Wang, XY,Chen, Y,Li, Z,&Wang, Z.(2002).Analysis of carbohydrates by capillary zone electrophoresis with on-capillary derivatization.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,25(4),589-600.
MLA Wang, XY,et al."Analysis of carbohydrates by capillary zone electrophoresis with on-capillary derivatization".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 25.4(2002):589-600.
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