A new type of capillary column for open-tubular electrochromatography
Zhao, YX; Zhao, R; Shangguan, DH; Liu, GQ
AbstractA new type of open-tubular C-18 ester-bonded electrochromatographic column was prepared with sol-gel technology, followed by an on-column octadecyl silylation reaction, Glycidoxypropyltrimethoxysilane, a widely used and important silane agent, was used as the sol-gel precursor to form a thin coating layer on the wall of the fused-silica capillary. The C-18 groups were introduced into the coating layer by on-column esterification reaction with stearic acid. The electrochromatography behavior of the column was evaluated in terms of the separation of peptides. A high efficiency of 4.8 x 10(5) plates/m was achieved for a basic pentapeptide using the C-18 ester-bonded column. In comparison with bare capillaries and glycidoxypropyltrimethoxysilane sol-gel-coated capillaries, the C-18 ester-bonded column showed the best separation of a mixture of seven pentapeptides under identical conditions of buffer, pH, and applied voltage.
KeywordOpen-tubular Capillary Electrochromatography Peptides Sol-gel Technology
Indexed BySCI
WOS IDWOS:000178154100021
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Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorLiu, GQ
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhao, YX,Zhao, R,Shangguan, DH,et al. A new type of capillary column for open-tubular electrochromatography[J]. ELECTROPHORESIS,2002,23(17):2990-2995.
APA Zhao, YX,Zhao, R,Shangguan, DH,&Liu, GQ.(2002).A new type of capillary column for open-tubular electrochromatography.ELECTROPHORESIS,23(17),2990-2995.
MLA Zhao, YX,et al."A new type of capillary column for open-tubular electrochromatography".ELECTROPHORESIS 23.17(2002):2990-2995.
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