ICCAS OpenIR
Solid phase derivatization of amino acids and peptides on alkaline silica gel support
Shangguan, DH; Zhao, YX; Han, HW; Zhao, R; Liu, GQ
2002-03-01
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN0251-0790
Volume23Issue:3Pages:385-387
AbstractA solid phase derivatization method of amino acids and peptides was described. Samples were added on alkaline silica gel cartridge and dried. The derivatization was carried out by passing the derivatization regent dissolved in anhydrous solvent through the cartridge slowly, then the cartridge was washed with a selected solvent, finally, the derivatives were eluted. The eluates were separated and detected by means of HPLC with fluorescence detector. Three derivatization reagents, 9-fluorenylmethyl chloroformate (FMOC-Cl), 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) and fluorescein isothiocyanate (FITC) were investigated with this method. The results showed that the method was suitable for derivatization of amino acids and some of peptides with FMOC-Cl because of its highly reacting activity and low polarity, the side-products of the FMOC-Cl with water were greatly decreased, the excess FMOC-Cl was eliminated completely, and the silica got support did not significantly affect the reaction rate and yield for amino acids. NBD-Cl was not suitable for this method because of the low yield of derivatization. FITC was also not suitable for this method because of its strong polarity.
KeywordSolid Phase Fluorescent Derivatization Fmoc-cl Alkaline Silica Gel Amino Acids Peptides
Indexed BySCI
Language英语
WOS IDWOS:000175465700012
PublisherHIGHER EDUCATION PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79406
Collection中国科学院化学研究所
Corresponding AuthorLiu, GQ
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Shangguan, DH,Zhao, YX,Han, HW,et al. Solid phase derivatization of amino acids and peptides on alkaline silica gel support[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2002,23(3):385-387.
APA Shangguan, DH,Zhao, YX,Han, HW,Zhao, R,&Liu, GQ.(2002).Solid phase derivatization of amino acids and peptides on alkaline silica gel support.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,23(3),385-387.
MLA Shangguan, DH,et al."Solid phase derivatization of amino acids and peptides on alkaline silica gel support".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 23.3(2002):385-387.
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