ICCAS OpenIR
Preparation and characteristics of high performance affinity chromatography medium used for the separation of antithrombin III
Zhao, YX; Shangguan, DH; Zhao, R; Liu, GQ
2002-02-01
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN0251-0790
Volume23Issue:2Pages:203-206
AbstractA high performance affinity chromatography medium used for separation of antithrombin III was prepared. Monodisperse poly (glycidyl methacrylate) beads (PGMA) were employed as the affinity support. Heparin was linked covalently to amino-PGMA-beads, which was prepared by amination of PGMA. The resultant affinity media present a minimal non-specific interaction with proteins and the protein recovery was 96. 9% and 94. 4% for antithrombin III and human serum albumin, respectively. Its affinity capacity for antithrombin was 1. 2 mg/g beads. The apparent dissociation constant between antithrombin II and immobilized heparin was 1. 96 X 10(-5) mol/L which was evaluated by front chromatography. The affinity medium was used to separate antithrombin E from human plasma.
KeywordHigh Performance Affinity Chromatography Heparin Antithrombin Iii
Indexed BySCI
Language英语
WOS IDWOS:000174163300010
PublisherHIGHER EDUCATION PRESS
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79396
Collection中国科学院化学研究所
Corresponding AuthorZhao, R
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhao, YX,Shangguan, DH,Zhao, R,et al. Preparation and characteristics of high performance affinity chromatography medium used for the separation of antithrombin III[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2002,23(2):203-206.
APA Zhao, YX,Shangguan, DH,Zhao, R,&Liu, GQ.(2002).Preparation and characteristics of high performance affinity chromatography medium used for the separation of antithrombin III.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,23(2),203-206.
MLA Zhao, YX,et al."Preparation and characteristics of high performance affinity chromatography medium used for the separation of antithrombin III".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 23.2(2002):203-206.
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