ICCAS OpenIR
Determination of andrographolide, deoxyandrographolide and neoandrographolide in the Chinese herb Andrographis paniculata by micellar electrokinetic capillary chromatography
Zhao, JX; Yang, GL; Liu, HX; Wang, DX; Song, XR; Chen, Y
2002-07-01
Source PublicationPHYTOCHEMICAL ANALYSIS
ISSN0958-0344
Volume13Issue:4Pages:222-227
AbstractIn this paper a micellar electrokinetic capillary chromatographic (MEKC) method has been developed for determining the active components (andrographolide, deoxyandrographolide and neoandrographolide) in water:ethanol extracts of the Chinese crude herb Andrographis paniculata and its preparations (Chuanxinlian and Xiaoyan Lidan tablets). The optimum separation conditions were 15 mm sodium dodecyl sulphate in 30 mm borate buffer (pH 9.5) with UV detection wavelength at 214 nm and a constant voltage of 16 kV. An HPLC method was employed in order to validate the MEKC method with respect to separation efficiency, sensitivity, linearity and repeatability. The two methods are shown to be complementary because of their different selectivity and thus are very suitable for cross-validation studies. The MEKC method is demonstrated to be more appropriate for the analysis of the active compounds in A. paniculata in that it is easier and less expensive to use and does not suffer from contamination of the chromatographic column. Copyright (C) 2002 John Wiley Sons, Ltd.
KeywordAndrographolide Deoxyandrographolide Neoandrographolide Micellar Electrokinetic Capillary Chromatography Andrographis Paniculata
DOI10.1002/pca.644
Indexed BySCI
Language英语
WOS IDWOS:000177131700007
PublisherJOHN WILEY & SONS LTD
Citation statistics
Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79356
Collection中国科学院化学研究所
Corresponding AuthorYang, GL
Affiliation1.Hebei Univ, Dept Chem, Baoding 071002, Peoples R China
2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 10080, Peoples R China
Recommended Citation
GB/T 7714
Zhao, JX,Yang, GL,Liu, HX,et al. Determination of andrographolide, deoxyandrographolide and neoandrographolide in the Chinese herb Andrographis paniculata by micellar electrokinetic capillary chromatography[J]. PHYTOCHEMICAL ANALYSIS,2002,13(4):222-227.
APA Zhao, JX,Yang, GL,Liu, HX,Wang, DX,Song, XR,&Chen, Y.(2002).Determination of andrographolide, deoxyandrographolide and neoandrographolide in the Chinese herb Andrographis paniculata by micellar electrokinetic capillary chromatography.PHYTOCHEMICAL ANALYSIS,13(4),222-227.
MLA Zhao, JX,et al."Determination of andrographolide, deoxyandrographolide and neoandrographolide in the Chinese herb Andrographis paniculata by micellar electrokinetic capillary chromatography".PHYTOCHEMICAL ANALYSIS 13.4(2002):222-227.
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