ICCAS OpenIR
Assembly and characterization of ternary SV-DNA-TMPyP complex Langmuir-Blodgett films
Chen, XD; Li, L; Liu, MH
2002-05-28
Source PublicationLANGMUIR
ISSN0743-7463
Volume18Issue:11Pages:4449-4454
AbstractTernary complex Langmuir-Blodgett (LB) films containing 1,1'-dioctadecyl-4,4'-bipyridinium (SV), deoxyribonucleic acid (DNA), and 5,10,15,20-tetrakis(4-N-methylpyridyl)porphine tetra(p-toluenesulfonate) (TMPyP) were assembled in two ways. One was assembled by depositing the complex monolayer of SV/DNA/TMPyP, which was in situ formed through the adsorption of aqueous subphase containing DNA and TMPyP onto the spreading SV monolayer (type I). Another was obtained by reacting the preformed SV/DNA LB film with TMPyP in aqueous solution (type II). A series of methods, such as surface pressure-area isotherms, atomic force microscopy (AFM), polarized UV-vis spectra, circular dichroism (CD), X-ray diffraction (XRD), and fluorescence lifetime measurements, were used to characterize these ternary LB films. The isotherm measurement indicated that stable complex monolayers could be formed at the air-water interface through the adsorption between SV and DNA or DNA/TMPyP. AFM showed different morphologies of type I and type II LB films. Through a series of characterizations on the LB films, it has been found that TMPyP can intercalate and electrostatically bind to DNA and orient differently in type I and type II LB films. Fluorescence lifetime measurements of the LB films indicated that in the type I LB film electron and/or energy transfer occurred predominantly between the SV and intercalated TMPyP, while in the type II LB film the transfer was predominantly between the SV and electrostatically bound TMPyP. Research showed that the deposition sequence or fabrication method plays an important role in the assembling of ternary LB systems. This method provides a way to fabricate DNA-based molecular assemblies in a controlled and precise manner.
DOI10.1021/la015718m
Indexed BySCI
Language英语
WOS IDWOS:000175801600042
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79348
Collection中国科学院化学研究所
Corresponding AuthorLiu, MH
Affiliation1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Lab Colloid & Interface Sci, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Chen, XD,Li, L,Liu, MH. Assembly and characterization of ternary SV-DNA-TMPyP complex Langmuir-Blodgett films[J]. LANGMUIR,2002,18(11):4449-4454.
APA Chen, XD,Li, L,&Liu, MH.(2002).Assembly and characterization of ternary SV-DNA-TMPyP complex Langmuir-Blodgett films.LANGMUIR,18(11),4449-4454.
MLA Chen, XD,et al."Assembly and characterization of ternary SV-DNA-TMPyP complex Langmuir-Blodgett films".LANGMUIR 18.11(2002):4449-4454.
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