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Chiral separation of amino acids derivatized with fluoresceine-5-isothiocyanate by capillary electrophoresis and laser-induced fluorescence detection using mixed selectors of beta-cyclodextrin and sodium taurocholate
Lu, XN; Chen, Y
2002-04-26
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume955Issue:1Pages:133-140
AbstractChiral separation of 20 pairs of amino acids derivatized with fluoresceine-5-isothiocyanate (FITC) by capillary electrophoresis and laser-induced fluorescence detection was studied using the mixture of beta-cyclodextrin (beta-CD) and sodium taurocholate (STC) as selector. Resolution was considerably superior to that obtained by using either P-CD or STC alone. The molar ratio of beta-CD to STC of about 2:3 was found to be critical to achieve maximum separation. At this beta-CD-to-STC ratio, chiral separation occurred at really low total concentration of beta-CD and STC (<0.1 mM). Other impacting factors were investigated including the total concentration of beta-CD and STC, pH, and capillary conditioning procedure between two successive runs. Using a running buffer of 80 mM borate containing 20 mM beta-CD and 30 mM STC at pH 9.3, all of the 20 pairs of FITC-amino acid enantiomers were baseline resolved. The resolutions of the most pairs of the amino acid enantiomers (17 of 20) were higher than 3.0, only three pairs gave a resolution lower than 3.0 but higher than 1.90 (beta-phenylserine, pSer). The highest resolution reached 14.58 (Glu). Two derivatives of beta-CD, 2-hydroxypropyl-beta-CD (HP-beta-CD) and heptakis(2,6-di-O-methyl)-beta-CD (DM-beta-CD) were also explored. HP-beta-CD showed similar cooperative effect with STC, while DM-beta-CD together with STC led to poorer chiral separation. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordEnantiomer Separation Derivitisation Electrophoresis Amino Acids Beta-cyclodextrin Sodium Taurocholate
Indexed BySCI
Language英语
WOS IDWOS:000175433800013
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:60[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79336
Collection中国科学院化学研究所
Corresponding AuthorChen, Y
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Grp 205, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Lu, XN,Chen, Y. Chiral separation of amino acids derivatized with fluoresceine-5-isothiocyanate by capillary electrophoresis and laser-induced fluorescence detection using mixed selectors of beta-cyclodextrin and sodium taurocholate[J]. JOURNAL OF CHROMATOGRAPHY A,2002,955(1):133-140.
APA Lu, XN,&Chen, Y.(2002).Chiral separation of amino acids derivatized with fluoresceine-5-isothiocyanate by capillary electrophoresis and laser-induced fluorescence detection using mixed selectors of beta-cyclodextrin and sodium taurocholate.JOURNAL OF CHROMATOGRAPHY A,955(1),133-140.
MLA Lu, XN,et al."Chiral separation of amino acids derivatized with fluoresceine-5-isothiocyanate by capillary electrophoresis and laser-induced fluorescence detection using mixed selectors of beta-cyclodextrin and sodium taurocholate".JOURNAL OF CHROMATOGRAPHY A 955.1(2002):133-140.
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