ICCAS OpenIR
Application of p-hydrazinebenzenesulfonic acid to carbohydrate determination by CE - Pre-capillary derivatization
Wang, XY; Chen, Y
2002
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN0251-0790
Volume23Issue:1Pages:34-37
Abstractp-Hydrazinebenzonesulfonic acid was explored as a novel pre-capillary labeling reagent for CE of mono- and disaccharides. The derivatization and the separation in borate buffer were investigated in detail. The derivatization took less than 10 min and the mass detection limit at 200 nm reaches 17.6 fmol of glucose, corresponding to concentration limit of 3.6 mumol/L- The baseline separation of 9 mono- and disaccharides could be achieved within 11 min, using a running buffer composed of 100 mmol/L borate at pH 10.24. This approach is also suitable for quantitation with linear coefficients higher than 0.992 at the concentrations between 0.1-9.1 mmol/L.
KeywordHigh Performance Capillary Electrophoresis Carbohydrate P-hydrazinebenzenesulfonic Acid Pre-capillary Derivatization
Indexed BySCI
Language英语
WOS IDWOS:000173834700010
PublisherHIGHER EDUCATION PRESS
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79290
Collection中国科学院化学研究所
Corresponding AuthorChen, Y
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wang, XY,Chen, Y. Application of p-hydrazinebenzenesulfonic acid to carbohydrate determination by CE - Pre-capillary derivatization[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2002,23(1):34-37.
APA Wang, XY,&Chen, Y.(2002).Application of p-hydrazinebenzenesulfonic acid to carbohydrate determination by CE - Pre-capillary derivatization.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,23(1),34-37.
MLA Wang, XY,et al."Application of p-hydrazinebenzenesulfonic acid to carbohydrate determination by CE - Pre-capillary derivatization".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 23.1(2002):34-37.
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