ICCAS OpenIR
Prolonged lifetime of the M intermediate in D96N-mutant bacteriorhodopsin films enhanced by diaza-15-crown-5
Liang, B; Li, BF; Zhang, JP; Jiang, L
2002
Source PublicationNEW JOURNAL OF CHEMISTRY
ISSN1144-0546
Volume26Issue:8Pages:1049-1053
AbstractIn order to get even longer M intermediate lifetime for the purpose of expanding the use of bacteriorhodopsin (BR) materials in optical applications, the combination of genetic engineering and the use of chemical additives was attempted. Different compositions of BR (wild type and D96N mutant)-PVA films with diaza-15-crown-5 (1,4,10-trioxa-7,13-diazacyclopentadecane) additive were prepared. Spectral and kinetic measurements were carried out at room temperature. It was observed that the additive contents that could be used in these films were limited. The highest BR : diaza-15-crown-5 molecular ratios were 1:250 and 1:150 for BRWT and BRD96N films, respectively. Further additions resulted in permanent damage to the purple membrane and a loss of photochromism. The kinetic curves of the M state decay for each film were fit, in a least-squares fashion, by a three-exponential function to obtain sufficiently small residuals. The fitting results indicate that for both types of BR molecules, the decay of the M state was slowed down gradually with increasing additive content. At a BRD96N : diaza-15-crown-5 molecular ratio of 1:150, the BR film had the longest lifetime and its photochromism could be observed for about one and a half hours. This indicates that the combination of genetic engineering and chemical additives is more efficient than using either of these two methods. The main reason for this significant prolongation may originate from the strong basic and H+ complexing properties of diaza-15-crown-5 and the change of the membrane surface charge caused by this additive.
DOI10.1039/b200827k
Indexed BySCI
Language英语
WOS IDWOS:000177009500016
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79288
Collection中国科学院化学研究所
Corresponding AuthorLi, BF
AffiliationCtr Mol Sci, Inst Chem, Lab Colloid & Interface Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Liang, B,Li, BF,Zhang, JP,et al. Prolonged lifetime of the M intermediate in D96N-mutant bacteriorhodopsin films enhanced by diaza-15-crown-5[J]. NEW JOURNAL OF CHEMISTRY,2002,26(8):1049-1053.
APA Liang, B,Li, BF,Zhang, JP,&Jiang, L.(2002).Prolonged lifetime of the M intermediate in D96N-mutant bacteriorhodopsin films enhanced by diaza-15-crown-5.NEW JOURNAL OF CHEMISTRY,26(8),1049-1053.
MLA Liang, B,et al."Prolonged lifetime of the M intermediate in D96N-mutant bacteriorhodopsin films enhanced by diaza-15-crown-5".NEW JOURNAL OF CHEMISTRY 26.8(2002):1049-1053.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liang, B]'s Articles
[Li, BF]'s Articles
[Zhang, JP]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liang, B]'s Articles
[Li, BF]'s Articles
[Zhang, JP]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liang, B]'s Articles
[Li, BF]'s Articles
[Zhang, JP]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.