ICCAS OpenIR
The divalent cation-induced DNA condensation studied by atomic force microscopy and spectra analysis
Sun, XG; Cao, EH; Zhang, XY; Liu, DG; Bai, CL
2002-03-01
Source PublicationINORGANIC CHEMISTRY COMMUNICATIONS
ISSN1387-7003
Volume5Issue:3Pages:181-186
AbstractThree types of DNA condensates in the presence of divalent metal ion (Mg, Mn and Cu) and EB simultaneously have been observed by atomic force microscopy (AFM). In the presence of Mg (II) and EB, DNA molecular exhibits three or four maggot-like branch crossing structures at the same point, which were constructed by some bead-like particles in each branch. However, in the presence of Cu (II) or Mn (II)-EB, DNA molecular forms a network or a semicircular structure, respectively. Their average height was 0.95 +/- 0.5 nm (Cu (II)-DNA-EB), 17+/- 5 nm (Mg (II)-DNA-EB) and 12 +/- 3 nm (Mn (II)-DNA-EB), respectively. However, DNA molecular or DNA-EB complex still remained a line double-stranded structure. The fluorescence, melting point and CD spectra indicate that the different morphologies induced by divalent metal ion (Mg (II), and Mn (II) and Cu (II)) are related to its binding position in molecular DNA, (C) 2002 Elsevier Science B.V, All rights reserved.
KeywordAtomic Force Microscopy Dna Condensation Divalent Metal Cation Dna Circular Dichroism
Indexed BySCI
Language英语
WOS IDWOS:000174593800002
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79264
Collection中国科学院化学研究所
Corresponding AuthorCao, EH
Affiliation1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Sun, XG,Cao, EH,Zhang, XY,et al. The divalent cation-induced DNA condensation studied by atomic force microscopy and spectra analysis[J]. INORGANIC CHEMISTRY COMMUNICATIONS,2002,5(3):181-186.
APA Sun, XG,Cao, EH,Zhang, XY,Liu, DG,&Bai, CL.(2002).The divalent cation-induced DNA condensation studied by atomic force microscopy and spectra analysis.INORGANIC CHEMISTRY COMMUNICATIONS,5(3),181-186.
MLA Sun, XG,et al."The divalent cation-induced DNA condensation studied by atomic force microscopy and spectra analysis".INORGANIC CHEMISTRY COMMUNICATIONS 5.3(2002):181-186.
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