ICCAS OpenIR
Difference in enzyme activity and conformation of glucose oxidase before and after purification
Dai, GL; Li, JR; Jiang, L
2002-04-01
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume24Issue:3-4Pages:171-176
AbstractEnzyme activity of commercial glucose oxidase was enhanced after purification through a strong anionic exchange resin. In order to get a better insight into this phenomenon, surface pressure-area (pi-A) isotherms and surface pressure-time (pi-t) isotherms was used to study the interaction and the absorption at different pH values of the subphases between octadecylamine and glucose oxidase purified by a styrene system quaternary ammonium type strongly basic anionic exchange resin. Circular dichroism (CD), electrophoresis and enzyme activity measurements were conducted to study these phenomena. A preliminary hypothesis has been suggested to explain why the enzyme activity of purified glucose oxidase was higher than that of the commercial one. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordGod Purification Surface Pressure-area Isotherms Surface Pressure-time Isotherms Octadecylamine
Indexed BySCI
Language英语
WOS IDWOS:000174555700001
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/79216
Collection中国科学院化学研究所
Affiliation1.Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Dai, GL,Li, JR,Jiang, L. Difference in enzyme activity and conformation of glucose oxidase before and after purification[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2002,24(3-4):171-176.
APA Dai, GL,Li, JR,&Jiang, L.(2002).Difference in enzyme activity and conformation of glucose oxidase before and after purification.COLLOIDS AND SURFACES B-BIOINTERFACES,24(3-4),171-176.
MLA Dai, GL,et al."Difference in enzyme activity and conformation of glucose oxidase before and after purification".COLLOIDS AND SURFACES B-BIOINTERFACES 24.3-4(2002):171-176.
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