ICCAS OpenIR
Fabrication and surface modification of macroporous poly(L-lactic acid) and poly(L-lactic-co-glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell culture
Yang, J; Shi, GX; Bei, JZ; Wang, SG; Cao, YL; Shang, QX; Yang, GG; Wang, WJ
2002-12-05
Source PublicationJOURNAL OF BIOMEDICAL MATERIALS RESEARCH
ISSN0021-9304
Volume62Issue:3Pages:438-446
AbstractThe fabrication and surface modification of a porous cell scaffold are very important in tissue engineering. Of most concern are high-density cell seeding, nutrient and oxygen supply, and cell affinity. In the present study, poly(L-lactic acid) and poly(L-lactic-co-glycolic acid) (70/30) cell scaffolds with different pore structures were fabricated. An improved method based on Archimedes' Principle for measuring the porosity of scaffolds, using a density bottle, was developed. Anhydrous ammonia plasma treatment was used to modify surface properties to improve the cell affinity of the scaffolds. The results show that hydrophilicity and surface energy were improved. The polar N-containing groups and positive charged groups also were incorporated into the sample surface. A low-temperature treatment was used to maintain the plasma-modified surface properties effectively. It would do help to the further application of plasma treatment technique. Cell culture results showed that pores smaller than 160 mum are suitable for human skin fibroblast cell growth. Cell seeding efficiency was maintained at above 99%, which is better than the efficiency achieved with the common method of prewetting by ethanol. The plasma-treatment method also helped to resolve the problem of cell loss during cell seeding, and the negative effects of the ethanol trace on cell culture were avoided. The results suggest that anhydrous ammonia plasma treatment enhances the cell affinity of porous scaffolds. Mass transport issues also have been considered. (C) 2002 Wiley Periodicals, Inc.
KeywordPolylactone Surface Modification Ammonia Plasma Treatment Human Skin Fibroblast Cells Tissue Engineering
DOI10.1002/jbm.10318
Indexed BySCI
Language英语
WOS IDWOS:000178014000015
PublisherJOHN WILEY & SONS INC
Citation statistics
Cited Times:268[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/78986
Collection中国科学院化学研究所
Corresponding AuthorWang, SG
Affiliation1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
2.Shanghai Tissue Engn Res & Dev Ctr, Shanghai 200011, Peoples R China
Recommended Citation
GB/T 7714
Yang, J,Shi, GX,Bei, JZ,et al. Fabrication and surface modification of macroporous poly(L-lactic acid) and poly(L-lactic-co-glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell culture[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH,2002,62(3):438-446.
APA Yang, J.,Shi, GX.,Bei, JZ.,Wang, SG.,Cao, YL.,...&Wang, WJ.(2002).Fabrication and surface modification of macroporous poly(L-lactic acid) and poly(L-lactic-co-glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell culture.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH,62(3),438-446.
MLA Yang, J,et al."Fabrication and surface modification of macroporous poly(L-lactic acid) and poly(L-lactic-co-glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell culture".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH 62.3(2002):438-446.
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