ICCAS OpenIR
Transient absorption and photovoltage study of' self-assembled bacteriorhodopsin/polycation multilayer films
Jussila, T; Li, ML; Tkachenko, NV; Parkkinen, S; Li, BF; Jiang, L; Lemmetyinen, H
2002-06-01
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume17Issue:6-7Pages:509-515
AbstractA series of organized (PDAC/PM)(n) (poly(diallyldimethylammonium chloride)/purple membrane) multilayer films were prepared by alternate adsorptions of positively charged PDAC polyelectrolyte and negatively charged purple membrane (PM). The kinetics of the photocycle of bacteriorhodopsin (bR) in PM was studied by flash photolysis and transient photovoltage methods. Although the orientation of the adsorbed bR depends on the pH of the PM suspension, the kinetics of the photo-induced reaction cycle in dehydrated films is independent of the deposition pH. In dry (PDAC/PM)(n) films the decay of the M intermediate to the initial bR state is multiexponential and delayed to several minutes for both orientations. A simultaneous two-exponential decay in millisecond time domain was observed at red wavelengths. The source of the red-shifted absorption is suggested to be the C-610 intermediate of the cis photocycle of bR. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordBacteriorhodopsin Polyelectrolyte Multilayer Film Self-assembly
Indexed BySCI
Language英语
WOS IDWOS:000175329400009
PublisherELSEVIER ADVANCED TECHNOLOGY
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/78920
Collection中国科学院化学研究所
Corresponding AuthorJussila, T
Affiliation1.Tampere Univ Technol, Inst Mat Chem, FIN-33101 Tampere, Finland
2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Lab Colloid & Interface Sci, Beijing 100101, Peoples R China
3.Univ Joensuu, Dept Biol, FIN-80101 Joensuu, Finland
Recommended Citation
GB/T 7714
Jussila, T,Li, ML,Tkachenko, NV,et al. Transient absorption and photovoltage study of' self-assembled bacteriorhodopsin/polycation multilayer films[J]. BIOSENSORS & BIOELECTRONICS,2002,17(6-7):509-515.
APA Jussila, T.,Li, ML.,Tkachenko, NV.,Parkkinen, S.,Li, BF.,...&Lemmetyinen, H.(2002).Transient absorption and photovoltage study of' self-assembled bacteriorhodopsin/polycation multilayer films.BIOSENSORS & BIOELECTRONICS,17(6-7),509-515.
MLA Jussila, T,et al."Transient absorption and photovoltage study of' self-assembled bacteriorhodopsin/polycation multilayer films".BIOSENSORS & BIOELECTRONICS 17.6-7(2002):509-515.
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