ICCAS OpenIR
Chiral separation of underivatized amino acids by ligand-exchange capillary electrophoresis using a copper(II)-L-lysine complex as selector
Lu, XN; Chen, Y; Guo, L; Yang, YF
2002-02-01
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume945Issue:1-2Pages:249-255
AbstractA ligand-exchange capillary electrophoretic method was explored, with (L)-lysine as the ligand and copper(II) as the central ion. Its applicability was demonstrated with underivatized aromatic amino acids, namely (D,L)-phenylalanine. (D,L)-tryptophan. (D,L)-tyrosine and (D,L)-beta-phenylserine. Optical resolutions of a single pair of amino acid enantiomers, and of mixed amino acids were obtained with a running buffer of 10 mM NH4Ac, 6.67 mM Cu(II) and 13.33 mM (L)-lysine, pH 7.0. Sodium dodecylsulfate (SDS) was shown to be necessary for simultaneous separation of the mixed amino acids. The resolution was found to increase with the concentration of the copper(II) complex at a copper(II)-to-lysine ratio of 1:2. If the total concentration of copper(II) and lysine was kept at 20 mM. decreasing the ratio of copper(II) to lysine caused a resolution loss of tryptophan, but a slight resolution improvement of the other three amino acids. The pH of buffer is another important factor controlling the separations. For all the studied amino acids, the optimum pH was 6.0. An interesting phenomenon was observed in this study. SDS induces precipitation at a concentration below 32 mM at room temperature (16+/-2 degreesC), possibly due to the formation of neutral substance from the SDS monomer and the copper(II)-lysine complex. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordEnantiomer Separation Ligand-exchange Capillary Electrophoresis Complexation Lysine Amino Acids
Indexed BySCI
Language英语
WOS IDWOS:000173609600020
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/78324
Collection中国科学院化学研究所
Corresponding AuthorChen, Y
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Grp 205, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Lu, XN,Chen, Y,Guo, L,et al. Chiral separation of underivatized amino acids by ligand-exchange capillary electrophoresis using a copper(II)-L-lysine complex as selector[J]. JOURNAL OF CHROMATOGRAPHY A,2002,945(1-2):249-255.
APA Lu, XN,Chen, Y,Guo, L,&Yang, YF.(2002).Chiral separation of underivatized amino acids by ligand-exchange capillary electrophoresis using a copper(II)-L-lysine complex as selector.JOURNAL OF CHROMATOGRAPHY A,945(1-2),249-255.
MLA Lu, XN,et al."Chiral separation of underivatized amino acids by ligand-exchange capillary electrophoresis using a copper(II)-L-lysine complex as selector".JOURNAL OF CHROMATOGRAPHY A 945.1-2(2002):249-255.
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