ESR studies on the superoxide radicals generated in photosystem II of higher plant
Liu, K; Sun, J; Liu, Y; Zhang, QY; Kuang, TY
AbstractSuperoxide radicals (O-2(radical anion)) produced in PSII particles separated from spinach has been investigated by spin trapping-electron spin resonance (ESR) technique. After bubbling with oxygen and incubating with tetracyanoethylene (TCNE) that acts as an inhibitor of superoxide dismutase (SOD) the experimental evidence of spin adduct of [DMPO-O2H](.) can be obtained by in situ ESR measurements in PSII particles. In contrast, adduct of O-2(radical anion) and DMPO produced under illumination is obviously decreased when SOD that usually performs as a scavenger of O-2(radical anion) is present. Furthermore, it is evident to find that the generation of O-2(radical anion) is positively correlated with the concentration of oxygen. The production of O-2(radical anion); is also pH dependent, and O-2(radical anion) concentration reaches maximum when pH is in the range of 6.0 similar to6.5. Either high, or low pH value will lead it to descend steeply. In Tris-HCl washed PS II particles donor-side ET inhibition can causes the production of O-2(radical anion) decreasing obviously. It can be concluded that the PSII that is active in splitting water and oxygen generation is also a reactive site responsible for the photoinduced O-2(radical anion) generation in thylakoid of higher plant. In another word, the formation of O-2(radical anion) is relevantly correlated to the electron transport activity of PS II.
KeywordSuperoxide Photosystem Ii Reactive Oxygen Species Spin Trapping Esr
Indexed BySCI
WOS IDWOS:000169596600022
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Liu, K,Sun, J,Liu, Y,et al. ESR studies on the superoxide radicals generated in photosystem II of higher plant[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2001,28(3):372-376.
APA Liu, K,Sun, J,Liu, Y,Zhang, QY,&Kuang, TY.(2001).ESR studies on the superoxide radicals generated in photosystem II of higher plant.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,28(3),372-376.
MLA Liu, K,et al."ESR studies on the superoxide radicals generated in photosystem II of higher plant".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 28.3(2001):372-376.
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