ICCAS OpenIR
Direct visualization of telomeric DNA loops in cells by AFM
Chen, A; Cao, EH; Sun, XG; Qin, JF; Liu, D; Wang, C; Bai, CL
2001-08-01
Source PublicationSURFACE AND INTERFACE ANALYSIS
ISSN0142-2421
Volume32Issue:1Pages:32-37
AbstractTelomeres are an essential nucleoprotein structure at the ends of all eukaryotic chromosomes. Studies on the structure and function of telomeres have received particular attention owing to their possible significance to ageing and cancer. In this work, human telomere from HeLa cells were isolated and purification by bio-gel P-2 column. Large duplex loops with a tail were first observed directly in the purified telomeric DNA species by atomic force microscopy (AFM). The lengths of the loop and tail are similar to2.5 +/- 0.5 and 2.0 +/- 1.5 kb, respectively. The AFM images also showed that the circular portion of loops is assumed to be a double-stranded structure according to its apparent height, but to be a triplex or tetraplex structure for the loop-tail junction. These results indicate that telomeres in human cells may end in large loops, which is an advantage in further studies on the structure and function of telomeres in cells and provides a new procedure for the study of telomere structure. Copyright (C) 2001 John Wiley & Sons, Ltd.
KeywordTelomeres Telomeric Dna T-loop G-quartet Atomic Force Microscopy
Indexed ByISTP ; SCI
Language英语
WOS IDWOS:000170551800007
PublisherJOHN WILEY & SONS LTD
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/77380
Collection中国科学院化学研究所
Corresponding AuthorCao, EH
Affiliation1.Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Chen, A,Cao, EH,Sun, XG,et al. Direct visualization of telomeric DNA loops in cells by AFM[J]. SURFACE AND INTERFACE ANALYSIS,2001,32(1):32-37.
APA Chen, A.,Cao, EH.,Sun, XG.,Qin, JF.,Liu, D.,...&Bai, CL.(2001).Direct visualization of telomeric DNA loops in cells by AFM.SURFACE AND INTERFACE ANALYSIS,32(1),32-37.
MLA Chen, A,et al."Direct visualization of telomeric DNA loops in cells by AFM".SURFACE AND INTERFACE ANALYSIS 32.1(2001):32-37.
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