ICCAS OpenIR
Analysis of trace amino acid neurotransmitters in hypothalamus of rats after exhausting exercise using microdialysis
Zhang, DM; Zhang, JM; Ma, WY; Chen, DY; Han, HW; Shu, HJ; Liu, GQ
2001-07-15
Source PublicationJOURNAL OF CHROMATOGRAPHY B
ISSN0378-4347
Volume758Issue:2Pages:277-282
AbstractA simple but effective coupling of microdialysis and capillary electrophoresis with laser induced fluorescence detection technique was applied to analysis of amino acid neurotransmitters in the hypothalamus of rats after acute exhausting exercise. The separation of amino acids was achieved using an uncoated fused-silica capillary (57 cm x 75 mum I.D.) with a buffer of 10 mM disodium tetraborate at pH 10 and an applied voltage of 12.5 kV. The detection limit was 10(-10) M for each amino acid. It is sufficiently sensitive and rapid for the determination of amino acids in a 5-mul Microdialysate. In comparison to pre-exercise, a significant increase in the levels of six hypothalamic amino acids (arginine, glycine, lysine, glutamic acid, alanine, gamma -amino-n-butyric acid) was found after exercise. These results demonstrate that the increase of metabolic amino acids in the hypothalamus of rats can be induced by exhausting exercise and suggests that amino acid neurotransmitters may play functional roles in the central effects of exercise. (C) 2001 Elsevier Science B.V. All rights reserved.
KeywordAmino Acid Neurotransmitters
Indexed BySCI
Language英语
WOS IDWOS:000169712400017
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/77366
Collection中国科学院化学研究所
Corresponding AuthorMa, WY
Affiliation1.Tsing Hua Univ, Dept Modern Appl Phys, Ctr Atom & Mol Sci, Beijing 100084, Peoples R China
2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, DM,Zhang, JM,Ma, WY,et al. Analysis of trace amino acid neurotransmitters in hypothalamus of rats after exhausting exercise using microdialysis[J]. JOURNAL OF CHROMATOGRAPHY B,2001,758(2):277-282.
APA Zhang, DM.,Zhang, JM.,Ma, WY.,Chen, DY.,Han, HW.,...&Liu, GQ.(2001).Analysis of trace amino acid neurotransmitters in hypothalamus of rats after exhausting exercise using microdialysis.JOURNAL OF CHROMATOGRAPHY B,758(2),277-282.
MLA Zhang, DM,et al."Analysis of trace amino acid neurotransmitters in hypothalamus of rats after exhausting exercise using microdialysis".JOURNAL OF CHROMATOGRAPHY B 758.2(2001):277-282.
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